https://scholars.lib.ntu.edu.tw/handle/123456789/458819
標題: | Mucin 15 is expressed in human placenta and suppresses invasion of trophoblast-like cells in vitro | 作者: | MING-KWANG SHYU MEI-CHUN LIN JIN-CHUNG SHIH CHIEN-NAN LEE JOHN HUANG Liao C.-H Huang I.-F Chen H.-Y MIN-CHUAN HUANG FON-JOU HSIEH |
公開日期: | 2007 | 卷: | 22 | 期: | 10 | 起(迄)頁: | 2723-2732 | 來源出版物: | Human Reproduction | 摘要: | Background: Trophoblast invasion is crucial for the development of normal placentas. Mucins are suggested to be involved in cancer invasion. However, the function of mucins in trophoblast invasion has never been reported. This study was to investigate the expression of mucin (MUC) 15 in human placenta and its role in trophoblast invasion. Methods: MUC15 mRNA in human tissues was analyzed by Northern blot. MUC15 mRNA and protein in human placenta were detected by real-time RT-PCR and Western blot, respectively. The distribution of MUC15 was revealed by immunohistochemistry. The effects of MUC15 on trophoblast invasion in vitro were analyzed by matrigel invasion assay in human choriocarcinoma JAR and JEG-3 cells. Results: MUC15 was expressed most highly in human placenta. MUC15 mRNA and protein increased with gestational age (P < 0.05, first versus third trimester). Immunohistochemistry showed that MUC15 protein was expressed by both cytotrophoblasts and syncytiotrophoblasts, especially at the apical membrane of syncytiotrophoblasts. In addition, MUC15 was found to be present in the glandular epithelium of the decidua. Overexpression of MUC15 substantially decreased matrigel invasion of JAR and JEG-3 cells by 87.5 ± 1.1 and 83.8 ± 5.7%, respectively, versus control, which was closely associated with an increase in mRNA expression of tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2. Knockdown of MUC15 with small interfering RNA significantly reversed these effects (P < 0.05). Conclusions: Differential expression of MUC15 in human placentas may play a critical role in the regulation of trophoblast invasion. ? The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/458819 | ISSN: | 0268-1161 | DOI: | 10.1093/humrep/dem249 | SDG/關鍵字: | messenger RNA; mucin; mucin 15; small interfering RNA; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2; unclassified drug; apical membrane; article; cell invasion; choriocarcinoma; controlled study; cytotrophoblast; decidua; epithelium; female; gestational age; human; human cell; human cell culture; human tissue; immunohistochemistry; in vitro study; Northern blotting; nucleotide sequence; placenta; protein analysis; protein expression; protein function; protein localization; reverse transcription polymerase chain reaction; syncytiotrophoblast; third trimester pregnancy; tissue distribution; trophoblast; Western blotting |
顯示於: | 醫學系 |
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