|Title:||Arsenic exposure and glucose intolerance/insulin resistance in estrogen-deficient female mice||Authors:||Huang C.-F.
|Issue Date:||2015||Publisher:||Public Health Services, US Dept of Health and Human Services||Journal Volume:||123||Journal Issue:||11||Start page/Pages:||1138-1144||Source:||Environmental Health Perspectives||Abstract:||
Background: Epidemiological studies have reported that the prevalence of diabetes in women > 40 years of age, especially those in the post menopausal phase, was higher than in men in areas with high levels of arsenic in drinking water. The detailed effect of arsenic on glucose metabolism/ homeostasis in the postmenopausal condition is still unclear. oBjectives: We investigated the effects of arsenic at doses relevant to human exposure from drinking water on blood glucose regulation in estrogen-deficient female mice. Methods: Adult female mice who underwent ovariectomy or sham surgery were exposed to drinking water contaminated with arsenic trioxide (0.05 or 0.5 ppm) in the presence or absence of 17β-estradiol supplementation for 2-6 weeks. Assays related to glucose metabolism were performed. results: Exposure of sham mice to arsenic significantly increased blood glucose, decreased plasma insulin, and impaired glucose tolerance, but did not induce insulin resistance. Blood glucose and insulin were higher, and glucose intolerance, insulin intolerance, and insulin resistance were increased in arsenic-treated ovariectomized mice compared with arsenic-treated sham mice. Furthermore, liver phosphoenol pyruvate carboxy kinase (PEPCK) mRNA expression was increased and liver glycogen content was decreased in arsenic-treated ovariectomized mice compared with arsenic-treated sham mice. Glucose-stimulated insulin secretion in islets isolated from arsenic-treated ovariectomized mice was also significantly decreased. Arsenic treatment significantly decreased plasma adiponectin levels in sham and ovariectomized mice. Altered glucose metabolism/ homeostasis in arsenic-treated ovariectomized mice was reversed by 17β-estradiol supplementation. conclusions: Our findings suggest that estrogen deficiency plays an important role in arsenicaltered glucose metabolism/homeostasis in females. ? 2015, Public Health Services, US Dept of Health and Human Services. All rights reserved.
|ISSN:||0091-6765||DOI:||10.1289/ehp.1408663||SDG/Keyword:||adiponectin; arsenic trioxide; estrogen; glucose; insulin; messenger RNA; phosphoenolpyruvate carboxykinase (pyrophosphate); adiponectin; ADIPOQ protein, human; arsenic; estradiol; estrogen; glucose blood level; insulin; animal experiment; animal model; arsenic poisoning; Article; controlled study; female; glucose intolerance; glucose metabolism; glucose regulation system; glycogen analysis; insulin resistance; male; mouse; nonhuman; oral glucose tolerance test; ovariectomy; priority journal; quantitative analysis; reverse transcription polymerase chain reaction; animal; blood; chemically induced; deficiency; drug effects; glucose blood level; glucose intolerance; liver; metabolism; Adiponectin; Animals; Arsenic; Blood Glucose; Estradiol; Estrogens; Female; Glucose Intolerance; Insulin; Insulin Resistance; Liver; Mice; Ovariectomy
|Appears in Collections:||醫學系|
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