Investigation into the pulmonary inflammopathology of exposure to nickel oxide nanoparticles in mice
Journal
Nanomedicine: Nanotechnology, Biology, and Medicine
Journal Volume
14
Journal Issue
7
Pages
2329-2339
Date Issued
2018
Author(s)
Bai, K.-J.
Chuang, K.-J.
Chen, J.-K.
Hua, H.-E.
Shen, Y.-L.
Liao, W.-N.
Lee, C.-H.
Chen, K.-Y.
Lee, K.-Y.
Hsiao, T.-C.
Pan, C.-H.
Ho, K.-F.
Chuang, H.-C.
Abstract
We investigated the effects of nickel oxide nanoparticles (NiONPs) on the pulmonary inflammopathology. NiONPs were intratracheally installed into mice, and lung injury and inflammation were evaluated between 1 and 28 days. NiONPs caused significant increases in LDH, total protein, and IL-6 and a decrease in IL-10 in the BALF and increases in 8-OHdG and caspase-3 in lung tissues at 24 h. Airway inflammation was present in a dose-dependent manner from the upper to lower airways at 24 h of exposure as analyzed by SPECT. Lung parenchyma inflammation and small airway inflammation were observed by CT after NiONP exposure. 8-OHdG in lung tissues had increased with formation of fibrosis at 28 days. Focal adhesion was the most important pathways identified at 24 h as determined by protemics, whereas glutathione metabolism was the most important identified at 28 days. Our results demonstrated the pulmonary inflammopathology caused by NiONPs based on image-to-biochemical approaches.
SDGs
Type
journal article