https://scholars.lib.ntu.edu.tw/handle/123456789/463672
標題: | Ectopic Pregnancy-Derived Human Trophoblastic Stem Cells Regenerate Dopaminergic Nigrostriatal Pathway to Treat Parkinsonian Rats | 作者: | Lee T.T.-Y Tsai C.-F Hsieh T.-H Chen J.-J.J Wang Y.-C Kao M.-C RUEY-MEEI WU Singh S Tsai E.-M Lee J.-N. |
公開日期: | 2012 | 卷: | 7 | 期: | 12 | 起(迄)頁: | e52491 | 來源出版物: | PLoS ONE | 摘要: | Background: Stem cell therapy is a potential strategy to treat patients with Parkinson's disease (PD); however, several practical limitations remain. As such, finding the appropriate stem cell remains the primary issue in regenerative medicine today. We isolated a pre-placental pluripotent stem cell from the chorionic villi of women with early tubal ectopic pregnancies. Our objectives in this study were (i) to identify the characteristics of hTS cells as a potential cell source for therapy; and (ii) to test if hTS cells can be used as a potential therapeutic strategy for PD. Methods and Findings: hTS cells expressed gene markers of both the trophectoderm (TE) and the inner cell mass (ICM). hTS cells exhibited genetic and biological characteristics similar to that of hES cells, yet genetically distinct from placenta-derived mesenchymal stem cells. All-trans retinoic acid (RA) efficiently induced hTS cells into trophoblast neural stem cells (tNSCs) in 1-day. Overexpression of transcription factor Nanog was possibly achieved through a RA-induced non-genomic c-Src/Stat3/Nanog signaling pathway mediated by the subcellular c-Src mRNA localization for the maintenance of pluripotency in tNSCs. tNSC transplantation into the lesioned striatum of acute and chronic PD rats not only improved behavioral deficits but also regenerated dopaminergic neurons in the nigrostriatal pathway, evidenced by immunofluorescent and immunohistological analyses at 18-weeks. Furthermore, tNSCs showed immunological advantages for the application in regenerative medicine. Conclusions: We successfully isolated and characterized the unique ectopic pregnancy-derived hTS cells. hTS cells are pluripotent stem cells that can be efficiently induced to tNSCs with positive results in PD rat models. Our data suggest that the hTS cell is a dynamic stem cell platform that is potentially suitable for use in disease models, drug discovery, and cell therapy such as PD. ? 2012 Lee et al. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/463672 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0052491 | SDG/關鍵字: | messenger RNA; protein tyrosine kinase; STAT3 protein; transcription factor NANOG; animal experiment; animal model; animal tissue; article; cell proliferation; cell regeneration; controlled study; dopaminergic nerve cell; ectopic pregnancy; enzyme localization; gene expression; human; human tissue; in vitro study; in vivo study; neural stem cell; neural stem cell transplantation; nigroneostriatal system; nonhuman; Parkinson disease; rat; signal transduction; stem cell transplantation; trophoblast |
顯示於: | 醫學系 |
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