Stem cells rescue cardiomyopathy induced by P. gingivalis-LPS via miR-181b
Journal
Journal of Cellular Physiology
Journal Volume
233
Journal Issue
8
Pages
5869-5876
Date Issued
2018
Author(s)
Chen, T.-S.
Battsengel, S.
Kuo, C.-H.
Pan, L.-F.
Lin, Y.-M.
Yao, C.-H.
Chen, Y.-S.
Lin, F.-H.
Kuo, W.-W.
Huang, C.-Y.
Abstract
Systemic inflammation induced by bacterial infection is one of several causative agents for cardiovascular disorders in patients with periodontal disease. Experimental results indicate that miRNAs play important roles in systemic inflammation induced by endotoxins. Further evidence states that stem cell based therapy shows potential in the treatment of inflammatory responses induced by sepsis. This study investigates if stem cells show protective effects on cardiomyocyte damage induced by porphyromonas gingivalis-LPS (Pg-LPS) through regulating miRNAs. H9c2 cardiomyoblasts and neonatal rat cardiomyocytes (NRCMs) were damaged using Pg-LPS in this study. Pg-LPS damaged H9c2 or NRCMs were then rescued using adipose-derived stem cells (ADSC). The experimental results reveal that Pg-LPS treatment is capable of inducing TLR4/NFκB axis activation, cell death signaling and IGF1R/PI3 K/Akt axis suppression. miR181b was downregulated in Pg-LPS damaged H9c2/NRCMs. All markers were improved in H9c2/NRCMs cocultured with ADSC. miR181b mimic and inhibitor confirmed that miR181b plays a central role in regulating the cardio protective effect on Pg-LPS damaged H9c2/NRCMs cocultured with ADSC. miR181b acts as potential therapeutic marker in cardiomyopathy induced by Pg-LPS. Transplantation of adipose-derived stem cells show potential in the treatment of cardiomyopathy induced by porphyromonas gingivalis endotoxin via regulation of miR181b. ? 2017 Wiley Periodicals, Inc.
Subjects
adipose-derived stem cells; cardiovascular disease; miR181b; periodontal disease
SDGs
Other Subjects
igf1r protein; immunoglobulin enhancer binding protein; lipopolysaccharide; microRNA; microRNA 181b; phosphatidylinositol 3 kinase; protein; protein kinase B; toll like receptor 4; unclassified drug; cardiotonic agent; immunoglobulin enhancer binding protein; lipopolysaccharide; microRNA; Tlr4 protein, rat; toll like receptor 4; adipose derived stem cell; animal cell; Article; cardiac muscle cell; cardiomyopathy; cell damage; cell death; cell protection; cell viability; comparative study; controlled study; down regulation; flow cytometry; H9c2(2-1) cell line; heart protection; human; newborn; nonhuman; Pi3K/Akt signaling; Porphyromonas gingivalis; priority journal; protein expression; rat; signal transduction; animal; cardiomyopathy; cell line; cell survival; genetics; inflammation; metabolism; microbiology; pathology; periodontal disease; Porphyromonas gingivalis; stem cell; Animals; Cardiomyopathies; Cardiotonic Agents; Cell Line; Cell Survival; Inflammation; Lipopolysaccharides; MicroRNAs; Myocytes, Cardiac; NF-kappa B; Periodontal Diseases; Porphyromonas gingivalis; Rats; Stem Cells; Toll-Like Receptor 4
Type
journal article