https://scholars.lib.ntu.edu.tw/handle/123456789/463927
標題: | Carbon encapsulated iron oxide nanoparticles surface engineered with polyethylene glycol-folic acid to induce selective hyperthermia in folate over expressed cancer cells | 作者: | Sadhasivam, S. Savitha, S. Wu, C.-J. Lin, F.-H. Stobi?ski, L. Lin, Feng-Huei |
關鍵字: | Carbon-arc; Folic acid; Hela cells; Hyperthermia; Iron oxide nanoparticles | 公開日期: | 2015 | 卷: | 480 | 期: | 1-2 | 起(迄)頁: | 8-14 | 來源出版物: | International Journal of Pharmaceutics | 摘要: | Carbon encapsulated iron oxide nanoparticles (CEIO-NPs) prepared by carbon arc method were successfully applied for in vitro magnetic hyperthermia. The CEIO-NPs were chemically oxidized and surface modified with PEG-FA for selective tumor localization in cancer cells that over expresses the folate receptors (FR+). The size, morphology, heating efficiency, biocompatibility and in vitro cell uptake of CEIO-PEG-FA NPs are extensively characterized. The as-prepared nanoparticles have generated quick heating (43-45 °C) upon exposure to an alternating magnetic field (AMF) with the saturation magnetization of 25 emu/g. The LDH cytotoxic assay demonstrated that the nanoparticle did not affect the viability of normal human fibroblast. The quantitative and cellular uptake studies by TEM confirmed the selective and increased uptake of CEIO-PEG-FA NPs when compared to the CEIO-nanoparticles. In conclusion, CEIO-PEG-FA NPs have the potential to induce magnetic hyperthermia in FR+ cells via the receptor mediated endocytosis uptake mechanism. ? 2015 Elsevier B.V. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/463927 | DOI: | 10.1016/j.ijpharm.2015.01.029 | SDG/關鍵字: | carbon; carbon encapsulated iron oxide nanoparticle; folic acid; iron nanoparticle; lactate dehydrogenase; macrogol; unclassified drug; folic acid; macrogol derivative; magnetite nanoparticle; Article; biocompatibility; cancer cell; cell viability; controlled study; cytotoxicity assay; endocytosis; female; fibroblast; heating; human; human cell; hyperthermia; in vitro study; magnetic field; nanoencapsulation; oxidation; particle size; priority journal; quantitative analysis; surface property; transmission electron microscopy; tumor localization; cell line; chemistry; comparative study; drug effects; HeLa cell line; metabolism; physiology; procedures; thermotherapy; Carbon; Cell Line; Endocytosis; Fibroblasts; Folic Acid; HeLa Cells; Humans; Hyperthermia, Induced; Magnetic Fields; Magnetite Nanoparticles; Particle Size; Polyethylene Glycols |
顯示於: | 醫學工程學研究所 |
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