Fabrication of keratin/fibroin membranes by electrospinning for vascular tissue engineering
Journal
Journal of Materials Chemistry B
Journal Volume
4
Journal Issue
2
Pages
237-244
Date Issued
2015
Author(s)
Abstract
Cardiovascular diseases (CVDs) are some of the leading causes of death and bypass surgery is one of the common treatment options for the critical CVD patients. There is no ideal material available for arterial bypass surgery. Herein, a fibroin and keratin porous membrane was prepared by electro-spinning and proposed for tissue-engineered vascular grafts (TEVGs). The purified fibroin (F) and keratin (K) were mixed in different weight ratios of 9-1 (FK91), 8-2 (FK82), and 7-3 (FK73) to obtain a membrane. The SEM images revealed that the electro-spinned membranes have a fibrous interconnected porous structure. The average diameter of the membrane F, FK91, FK82 and FK73 was 5.74 ± 1.04, 4.20 ± 1.19, 2.94 ± 0.81 and 2.27 ± 0.65 μm, respectively. The ultimate tensile strength (UTS) of F, FK91, FK82 and FK73 was 2.09 ± 0.06, 2.02 ± 0.06, 1.81 ± 0.10 and 1.74 ± 0.12 MPa, respectively. The contact angle of F, FK91, FK82 and FK73 was 72.55 ± 0.55°, 66.39 ± 0.90°, 43.47 ± 0.04°and 33.65 ± 2.83°, respectively. The wettability results were in agreement with those of the cell adhesion to the electro-spinning membranes. The attached HUVECs on the developed membranes showed no cytotoxicity. The immunocytochemistry staining and qPCR analysis showed that the phenotype of the keratin-fibroin membrane was not altered. The results of the ultimate tensile strength, cell adhesion and microstructure revealed that FK82 is similar to native vessels and could be considered as a potential material for TEVGs. ? 2016 The Royal Society of Chemistry.
SDGs
Other Subjects
Cardiovascular surgery; Cell adhesion; Diseases; Keratin; Membranes; Patient treatment; Polymerase chain reaction; Surgery; Tensile strength; Tissue; Tissue engineering; Average diameter; Cardio-vascular disease; Immunocytochemistry; Interconnected porous structure; Potential materials; Tissue engineered vascular grafts; Ultimate tensile strength; Vascular tissue engineering; Fibrous membranes
Type
journal article