https://scholars.lib.ntu.edu.tw/handle/123456789/463937
標題: | Development of a dual-functional Pt-Fe-HAP magnetic nanoparticles application for chemo-hyperthermia treatment of cancer | 作者: | Tseng, C.-L. Chang, K.-C. Yeh, M.-C. Yang, K.-C. Tang, T.-P. Lin, F.-H. Lin, Feng-Huei |
關鍵字: | Chemotherapy; Hydroxyapatite (HAP); Hyperthermia; Magnetic particle; Platinum | 公開日期: | 2014 | 卷: | 40 | 期: | 4 | 起(迄)頁: | 5117-5127 | 來源出版物: | Ceramics International | 摘要: | Lung cancer is a harmful form of cancer; chemotherapy is the main methodology for treating it, despite continuing problems such as severe side effects. For the reduction of side effects, hydroxyapatite (HAP) has been investigated as a drug carrier recently. Moreover, hyperthermia has been reported to be an effective cancer treatment modality. In order to develop an effective agent for lung cancer treatment, dual-functional nanoparticles made from HAP with iron and platinum ions incorporation (Pt-Fe-HAP) were developed for chemo-hyperthermia application. Variant HAP were synthesized and analyzed in this study. The crystallization and chemical composition were examined by X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy. Fe 2+ and Pt2+ content was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES). The biocompatibility and anticancer effects of Pt-Fe-HAP were assessed using 3T3 cells via a WST-1 assay. The effect of hyperthermia on the treatment of Sprague Dawley (SD) rat fibroblast and human lung adenocarcinoma (A549) cells under a magnetic field was evaluated by a lactate dehydrogenase (LDH) assay. Overall, we have determined Pt-Fe-HAP could be prepared by a co-precipitation method with Fe 2+/Pt2+ incorporation. The platinum and iron contents were 3.69 wt% and 12.20 wt%, respectively. The hysteresis curves showed that the Pt-Fe-HAP possessed ferromagnetic properties at low magnetic fields. The water temperature could be raised to 46 C when the Pt-Fe-HAP suspension was treated in a magnetic field for 6 min. We also confirmed that the extraction solution of Pt-Fe-HAP was nontoxic, but the direct culture of 3T3 cells with these nanoparticles was harmful. Finally, the results of the LDH assay revealed Pt-Fe-HAP was highly toxic to A549 cells after magnetic field treatment under hyperthermia but no damage to fibroblast cells was observed. The magnetic Pt-Fe-HAP nanoparticles show the potential to be a dual agent to treat cancer cells by chemo-hyperthermia therapy. ? 2013 Elsevier Ltd and Techna Group S.r.l. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/463937 | DOI: | 10.1016/j.ceramint.2013.09.137 |
顯示於: | 醫學工程學研究所 |
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