https://scholars.lib.ntu.edu.tw/handle/123456789/463970
Title: | In vitro feasibility study of the use of a magnetic electrospun chitosan nanofiber composite for hyperthermia treatment of tumor cells | Authors: | Lin, T.-C. Lin, F.-H. Lin, J.-C. Lin, Feng-Huei |
Keywords: | Cancer therapy; Electrospinning; Hyperthermia; Magnetic electrospun nanofiber matrix; Magnetic nanoparticles | Issue Date: | 2012 | Journal Volume: | 8 | Journal Issue: | 7 | Start page/Pages: | 2704-2711 | Source: | Acta Biomaterialia | Abstract: | Hyperthermia has been reported to be an effective cancer treatment modality, as tumor cells are more temperature-sensitive than their normal counterparts. Since the ambient temperature can be increased by placing magnetic nanoparticles in an alternating magnetic field it has become of interest to incorporate these magnetic nanoparticles into biodegradable nanofibers for possible endoscopic hyperthermia treatment of malignant tumors. In this preliminary investigation we have explored various characteristics of biodegradable electrospun chitosan nanofibers containing magnetic nanoparticles prepared by different methods. These methods included: (1) E-CHS-Fe 3O4, with electrospun chitosan nanofibers directly immersed in a magnetic nanoparticle solution; (2) E-CHS-Fe2+, with the electrospun chitosan nanofibers initially immersed in Fe+2/ Fe+3 solution, followed by chemical co-precipitation of the magnetic nanoparticles. The morphology and crystalline phase of the magnetic electrospun nanofiber matrices were determined by scanning electron microscopy, transmission electron microscopy, selected area electron diffraction, and X-ray diffraction spectroscopy. The magnetic characteristics were measured using a superconducting quantum interference device. The heating properties of these magnetic electrospun nanofiber matrices in an alternating magnetic field were investigated at a frequency of 750 kHz and magnetic intensity of 6.4 kW. In vitro cell incubation experiments indicated that these magnetic electrospun nanofiber matrices are non-cytotoxic and can effectively reduce tumor cell proliferation upon application of a magnetic field. ? 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/463970 | DOI: | 10.1016/j.actbio.2012.03.045 | SDG/Keyword: | Cell proliferation; Chitosan; Diseases; Drug delivery; Electron diffraction; Electrospinning; High resolution transmission electron microscopy; Hyperthermia therapy; Magnetic fields; Magnetite; Nanofibers; Nanomagnetics; Precipitation (chemical); Scanning electron microscopy; SQUIDs; Tumors; X ray diffraction; Cancer therapy; Chitosan nanofibers; Electrospun nanofibers; Electrospuns; Hyperthermia; Hyperthermia treatments; In-vitro; Magnetic electrospun nanofiber matrix; Nanofiber matrix; Tumour cells; Nanoparticles; chitosan; iron complex; magnetic nanoparticle; magnetite nanoparticle; nanofiber; article; cancer therapy; cell proliferation; electron diffraction; electrospinning; feasibility study; heating; human; human cell; hyperthermic therapy; in vitro study; magnetic field; malignant neoplastic disease; precipitation; priority journal; quantum mechanics; scanning electron microscopy; temperature sensitivity; transmission electron microscopy; tumor cell; X ray diffraction |
Appears in Collections: | 醫學工程學研究所 |
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