|Title:||Facile synthesis of hollow mesoporous hydroxyapatite nanoparticles for intracellular bio-imaging||Authors:||Wu, K.C.-W.
KEVIN CHIA-WEN WU
|Issue Date:||2011||Journal Volume:||7||Journal Issue:||6||Start page/Pages:||926-931||Source:||Current Nanoscience||Abstract:||
The effects of several critical factors including the amount of EG, the reaction time, the concentrations of Ca(CH3COO)2 and NaHCO3, the concentration of H3PO4, and the amount of acetic acid were investigated for synthesizing hollow mesoporous hydroxyapatite nanoparticles (hm-HANPs). We optimized the reaction conditions as follows: the concentration of Ca(CH3COO)2 and NaHCO3 was 0.3 M, the ratio of Ca(CH3COO)2 and NaHCO3 to ethylene glycol (EG) was 1:5, the reaction time for the formation of hydroxyapatite was 3 hr, the concentration of H3PO4 was 0.01 M, and the ratio of hm-HANP solution to acetic acid was 1:3.125. Monodispersed hm-HANPs with a defined particle size could be obtained. A fluorescent dye named fluorescein isothiocyanate (FITC) was successfully linked to the surface of hm-HANPs for the bioimaging of hm-HANPs in living breast cancer cells (BT-20). The FITC-hm-HANPs were taken up by the cells and stayed in the cytoplasm with high biocompatibility. The information obtained in this study will benefit the synthesis of hydroxyapatite nanostructures for many biomedical-related applications. ? 2011 Bentham Science Publishers.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/463978||DOI:||10.2174/157341311798220763||SDG/Keyword:||Bio-imaging; Breast cancer cells; Critical factors; Facile synthesis; Fluorescein isothiocyanate; Fluorescent dyes; Hollow; Intracellular; Mesoporous; Mono-dispersed; Reaction conditions; Acetic acid; Apatite; Biocompatibility; Calcium; Cells; Cytology; Ethylene; Ethylene glycol; Imaging techniques; Medical imaging; Mesoporous materials; Nanoparticles; pH; Synthesis (chemical); Hydroxyapatite; acetic acid; bicarbonate; calcium acetate; calcium carbonate; ethylene glycol; fluorescein isothiocyanate; hydroxyapatite; hydroxyapatite nanoparticle; nanoparticle; nanoshell; phosphate; phosphoric acid; unclassified drug; article; biocompatibility; breast cancer; cancer cell; chemical structure; concentration (parameters); cytoplasm; cytotoxicity; dispersion; human; human cell; molecular imaging; morphology; particle size; priority journal; reaction time; synthesis
|Appears in Collections:||醫學工程學研究所|
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