Effects of composite substrate microstructure on fibroblast morphology and migration
Journal
ASME 2011 Summer Bioengineering Conference
Journal Issue
PARTS A AND B
Pages
959-960
Date Issued
2011
Author(s)
Abstract
Many studies have focused on the effects of substrate rigidity on cell traction, migration, and differentiation [1–3]. Most cells are known to migrate toward the stiffer substrate, a phenomenon known as durotaxis. Recent reports have also demonstrated the ‘depth-sensing’ ability of cells on soft hydrogels where cell behaviors on thin gels are more similar to those on stiffer substrates [4–5]. Taking advantage of the high fidelity of microfabrication and soft lithography products, we created novel composite substrates composed of a top layer of collagen hydrogel and an underlying microstructure of silicon elastomer. We hypothesize that cells can sense the underlying microstructures and regulate cell translocation and morphology accordingly.
SDGs
Type
conference paper