https://scholars.lib.ntu.edu.tw/handle/123456789/465288
Title: | Regulation of vascular endothelial growth factor secretion in human meningioma cells | Authors: | JUI-CHANG TSAI Hsiao Y.-Y. LEE-JENE TENG CHIA-TUNG SHUN CHIN-TIN CHEN Goldman C.K. Kao M.-C. |
Issue Date: | 1999 | Journal Volume: | 98 | Journal Issue: | 2 | Start page/Pages: | 111-117 | Source: | Journal of the Formosan Medical Association | Abstract: | Previously, we induced vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) secretion in glioma cell lines by using physiologic concentrations of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), or platelet-derived growth factor-BB (PDGF-BB). We hypothesized that VEGF/VPF might enhance the blood supply required for the unregulated growth of tumors, and that it acts as the central mediator of tumor angiogenesis. The objective of this study was to determine whether the expression of VEGF/VPF by meningiomas is regulated by growth factors or sex hormones. By means of an enzyme-linked immunosorbent assay of CH-157MN meningioma cell supernatants, we demonstrated that EGF and bFGF similarly induce VEGF secretion by CH-157MN meningioma cells. At the maximum concentrations of EGF (50 ng/mL) and bFGF (50 ng/mL) used in this study, VEGF secretion was induced to 140% to 160% above baseline constitutive secretion. PDGF-BB homodimer did not enhance VEGF secretion significantly. Estradiol (up to 10-7 mol/L), progesterone (up to 10-5 mol/L), or testosterone (up to 10-5 mol/L) did not stimulate or inhibit VEGF secretion in CH-157MN meningioma cells (p > 0.05). Furthermore, we demonstrated that dexamethasone decreased VEGF secretion to 32% of baseline constitutive secretion. This might explain the effect of corticosteroids in alleviating peritumoral brain edema in meningiomas. These results suggest that VEGF secretion in CH-157MN meningioma cells is mainly regulated by growth factors and corticosteroids, but not by sex hormones. Understanding the regulation of VEGF/VPF secretion in meningiomas might contribute to the development of a new therapeutic strategy. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033039040&partnerID=40&md5=d58e9f62c1f0cc85d167b5536a09d68b https://scholars.lib.ntu.edu.tw/handle/123456789/465288 |
ISSN: | 0929-6646 | SDG/Keyword: | basic fibroblast growth factor; corticosteroid; dexamethasone; epidermal growth factor; estradiol; mifepristone; platelet derived growth factor; progesterone; sex hormone; testosterone; vasculotropin; angiogenesis; article; cancer cell culture; cell proliferation; concentration response; controlled study; hormonal regulation; human; human cell; meningioma; pathophysiology; protein determination; protein expression; protein induction; protein synthesis regulation; tumor vascularization; Dexamethasone; Endothelial Growth Factors; Enzyme-Linked Immunosorbent Assay; Estradiol; Female; Glucocorticoids; Gonadal Steroid Hormones; Growth Substances; Humans; Lymphokines; Meningeal Neoplasms; Meningioma; Middle Aged; Platelet-Derived Growth Factor; Progesterone; Testosterone; Tumor Cells, Cultured; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors |
Appears in Collections: | 醫學系 |
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