https://scholars.lib.ntu.edu.tw/handle/123456789/465308
標題: | Chitosan-hyaluronan: promotion of mucociliary differentiation of respiratory epithelial cells and development of olfactory receptor neurons | 作者: | Huang T.-W Li S.-T Tai-Horng Young |
關鍵字: | chitosan; Chronic rhinosinusitis; hyaluronan; olfactory neuroepithelium; respiratory epithelium | 公開日期: | 2019 | 卷: | 47 | 期: | 1 | 起(迄)頁: | 564-570 | 來源出版物: | Artificial Cells, Nanomedicine and Biotechnology | 摘要: | Developing a biomaterial that promotes regeneration of both respiratory epithelium (RE) and olfactory neuroepithelium (ON) improves the surgical outcome of endoscopic sinus surgery. Although chitosan (CS) inhibits mucociliary differentiation of RE, it has been reported to regenerate ON. In addition, hyaluronic acid (HA) has been demonstrated to promote regeneration of RE. Whether the composite CS + HA would simultaneously benefit RE and ON remains unexplored. Human nasal respiratory epithelial cells (RECs) and olfactory neuroepithelial cells (ONCs) are respectively obtained from the RE and the ON. They are cultured in vitro and divided into groups undergoing four treatments, control, CS, HA, and CS + HA and assessed by scanning electron microscope, immunocytochemistry, and Western blots following indicated growth conditions. RECs keep polygonal morphology with mucociliary differentiation in the CS + HA group. The levels of E-cadherin, zonula occludens-1, mucin 5AC, and forkhead box protein J1 are significantly higher in the CS + HA group than in the CS alone group. In addition, ONCs express lower cytokeratin 18 (CK18) and higher olfactory marker protein (OMP) in the CS + HA group than in HA alone group. ONCs express more signal transduction apparatuses, adenylate cyclase 3, in CS and CS + HA groups than in HA and controls. Chitosan-hyaluronan plays a part in promoting differentiation of ORNs and facilitating mucociliary differentiation of RECs. This composite is a promising biomaterial for the sinonasal application. ? 2019, ? 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/465308 | ISSN: | 2169-1401 | DOI: | 10.1080/21691401.2019.1579732 | SDG/關鍵字: | Cells; Chitosan; Proteins; Scanning electron microscopy; Signal transduction; Transplantation (surgical); Adenylate cyclase; Chronic rhinosinusitis; Endoscopic Sinus Surgery; Hyaluronan; Immunocytochemistry; olfactory neuroepithelium; Olfactory receptor neurons; Respiratory epitheliums; Hyaluronic acid; adenylate cyclase; adenylate cyclase 3; biomaterial; chitosan; cytokeratin 18; forkhead box protein J1; forkhead transcription factor; glyceraldehyde 3 phosphate dehydrogenase; hyaluronic acid; mucin 5AC; olfactory marker protein; protein ZO1; unclassified drug; uvomorulin; chitosan; hyaluronic acid; airway epithelium cell; Article; cell differentiation; cell growth; cell structure; ciliated epithelium cell; controlled study; human; human cell; human tissue; immunocytochemistry; in vitro study; neuroepithelium; neuroepithelium cell; olfactory receptor neuron; protein expression level; respiratory epithelium; signal transduction; Western blotting; cell culture; cell differentiation; chronic disease; drug effect; nose mucosa; olfactory receptor neuron; pathology; rhinitis; Cell Differentiation; Cells, Cultured; Chitosan; Chronic Disease; Humans; Hyaluronic Acid; Nasal Mucosa; Olfactory Receptor Neurons; Rhinitis; Signal Transduction |
顯示於: | 醫學工程學研究所 |
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