Increased cell detachment ratio of mesenchymal-type lung cancer cells on pH-responsive chitosan through the 3 integrin
Journal
Marine Drugs
Journal Volume
17
Journal Issue
12
Date Issued
2019
Author(s)
Abstract
Chitosan is sensitive to environmental pH values due to its electric property. This study investigates whether the pH-responsive chitosan assay can provide a simple method to evaluate the aggressive behavior of cancer cells with cell detachment ratio. The epithelial-mesenchymal transition (EMT) is induced with transforming growth factor-β1 (TGF-β1) in the human non-small cell lung cancer cell line (A549). EMT-induced cells and untreated cells are cultured on chitosan substrates at pH 6.99 for 24 h, followed by pH 7.65 for 1 h. The cell detachment ratio (CDR) on pH-responsive chitosan rises with an increasing of the TGF-β1 concentration. The protein array reveals that the expression levels of the α2, α3, α5, β2, and β3 integrins are higher in EMT-induced A549 cells than in untreated cells. A further inhibition assay shows that adding β3 integrin blocking antibodies significantly decreases the CDR of EMT-induced cells from 32.7 ± 5.7% to 17.8 ± 2.1%. The CDR of mesenchymal-type lung cancer cells increases on pH-responsive chitosan through the β3 integrin. Notably, the CDR can be theoretically predicted according to the individual CDR on the pH-responsive chitosan surface, irrespective of heterogeneous cell mixture. The pH-responsive chitosan assay serves as a simple in vitro model to investigate the aggressive behavior of lung cancer including the heterogeneous cell population. ? 2019 MDPI AG. All rights reserved.
Subjects
Cell detachment ratio; Epithelial-mesenchymal transition; Integrins; Lung cancer; pH-responsive chitosan; Transforming growth factor-β1
SDGs
Other Subjects
alpha2 integrin; alpha3 integrin; alpha5 integrin; beta3 integrin; blocking antibody; CD18 antigen; chitosan; transforming growth factor beta1; beta3 integrin; chitosan; ITGB3 protein, human; TGFB1 protein, human; transforming growth factor beta1; A-549 cell line; Article; cancer growth; cell culture; cell detachment ratio; cell function; cell metabolism; cell population; controlled study; enzyme inhibition assay; epithelial mesenchymal transition; human; human cell; in vitro study; lung cancer cell line; pH; protein expression; protein microarray; bioassay; cell adhesion; cell motion; chemistry; epithelial mesenchymal transition; lung tumor; metabolism; non small cell lung cancer; pathology; pH; procedures; signal transduction; A549 Cells; Biological Assay; Carcinoma, Non-Small-Cell Lung; Cell Adhesion; Cell Movement; Chitosan; Epithelial-Mesenchymal Transition; Humans; Hydrogen-Ion Concentration; Integrin beta3; Lung Neoplasms; Signal Transduction; Transforming Growth Factor beta1
Type
journal article