https://scholars.lib.ntu.edu.tw/handle/123456789/465398
標題: | Gene expression of human lung cancer cell line cl1-5 in response to a direct current electric field | 作者: | Huang C.-W Chen H.-Y Yen M.-H Chen J.J.W Tai-Horng Young Cheng J.-Y. |
公開日期: | 2011 | 卷: | 6 | 期: | 10 | 來源出版物: | PLoS ONE | 摘要: | Background: Electrotaxis is the movement of adherent living cells in response to a direct current (dc) electric field (EF) of physiological strength. Highly metastatic human lung cancer cells, CL1-5, exhibit directional migration and orientation under dcEFs. To understand the transcriptional response of CL1-5 cells to a dcEF, microarray analysis was performed in this study. Methodology/Principal Findings: A large electric-field chip (LEFC) was designed, fabricated, and used in this study. CL1-5 cells were treated with the EF strength of 0mV/mm (the control group) and 300mV/mm (the EF-treated group) for two hours. Signaling pathways involving the genes that expressed differently between the two groups were revealed. It was shown that the EF-regulated genes highly correlated to adherens junction, telomerase RNA component gene regulation, and tight junction. Some up-regulated genes such as ACVR1B and CTTN, and some down-regulated genes such as PTEN, are known to be positively and negatively correlated to cell migration, respectively. The protein-protein interactions of adherens junction-associated EF-regulated genes suggested that platelet-derived growth factor (PDGF) receptors and ephrin receptors may participate in sensing extracellular electrical stimuli. We further observed a high percentage of significantly regulated genes which encode cell membrane proteins, suggesting that dcEF may directly influence the activity of cell membrane proteins in signal transduction. Conclusions/Significance: In this study, some of the EF-regulated genes have been reported to be essential whereas others are novel for electrotaxis. Our result confirms that the regulation of gene expression is involved in the mechanism of electrotactic response. ? 2011 Huang et al. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/465398 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0025928 | SDG/關鍵字: | cell membrane protein; ephrin receptor; platelet derived growth factor receptor; transcriptome; ACVR1B gene; article; cancer cell; cell junction; cell migration; cellular distribution; controlled study; CTTN gene; down regulation; electric field; gene control; gene expression regulation; human; human cell; lab on a chip; lung cancer; microarray analysis; nucleotide sequence; oncogene; protein domain; protein protein interaction; PTEN gene; signal transduction; unindexed sequence; upregulation; cell motion; DNA microarray; electricity; intracellular space; lung tumor; metabolism; microfluidic analysis; pathology; protein transport; tumor cell line; Cell Line, Tumor; Cell Movement; Electricity; Humans; Intracellular Space; Lung Neoplasms; Microfluidic Analytical Techniques; Oligonucleotide Array Sequence Analysis; Protein Transport; Signal Transduction; Transcriptome |
顯示於: | 醫學工程學研究所 |
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