https://scholars.lib.ntu.edu.tw/handle/123456789/465410
標題: | Multilayered polyplexes with the endosomal buffering polycation in the core and the cell uptake-favorable polycation in the outer layer for enhanced gene delivery | 作者: | Ke J.-H Tai-Horng Young |
關鍵字: | Gene therapy; Gene vehicle; Polycation; Polyelectrolyte multilayer | 公開日期: | 2010 | 卷: | 31 | 期: | 35 | 起(迄)頁: | 9366-9372 | 來源出版物: | Biomaterials | 摘要: | In the present study, quaternary polyplexes were prepared by sequential addition of polycations (polyethylenimine (PEI) or poly (N-(8-aminooctyl)-acrylamide) (P8Am)) for loading pDNA into the core polyplexes and poly (acrylic acid) (PAA) for reversing charges to deposit additional polycation (PEI or P8Am) layer. It was found the cytotoxicity and cellular uptake expression of PEI core polyplexes could be improved by coating a cell uptake-favorable P8Am layer. Conversely, P8Am could not facilitate endosomal release through the proposed proton sponge effect so the PEI core was required for the P8Am-coated quaternary polyplexes to ensure efficient transfection. Consequently, an efficient and safe non-viral gene vehicle was constructed by layer-by-layer deposition, using alternate polyanion and polycation with required functionalities to overcome the obstacles met in the process of transfection. Maximum transfection activity with minimal toxicity was observed when the quaternary polyplex of pDNA/PEI/PAA/P8Am was prepared at a weight ratio of 1/1.5/3/5. Conversely, the same composition in different position such as the cell-favorable P8Am core was externally deposited with the endosome lytic moiety, PEI showed high toxicity and low efficiency. This indicates the pDNA/PEI/PAA/P8Am sequence for a quaternary polyplex is as important as the functional polymer selection for designing safe and reliable gene delivery vehicles. We demonstrate here that gene delivery efficiency may be improved by increasing the uptake level and the endosomal buffering release through an additional layer of cell uptake-favorable polycations associated with the core polycations possessing endosomal release ability. ? 2010 Elsevier Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/465410 | ISSN: | 0142-9612 | DOI: | 10.1016/j.biomaterials.2010.08.066 | SDG/關鍵字: | Acrylamides; Cell uptake; Cellular uptake; Endosomal release; Endosomes; Gene Delivery; Layer by layer deposition; Multi-layered; Nonviral; Outer layer; Polyacrylic acids; Polyanions; Polycations; Polyelectrolyte multilayer; Polyethylenimines; Polyplexes; Proton sponge; Required functionalities; Sequential addition; Transfection activity; Weight ratios; Amides; Carboxylic acids; Deposition; Functional polymers; Gene transfer; Hydrogels; Multilayers; Organic acids; Polyelectrolytes; Toxicity; Vehicles; Gene therapy; polycation; article; cancer cell; cytotoxicity; endosome; gene expression; gene targeting; human; human cell; internalization; molecular weight; priority journal; Cell Survival; Gene Therapy; Gene Transfer Techniques; Hela Cells; Hep G2 Cells; Humans; Polyamines; Polyethyleneimine; Polymers |
顯示於: | 醫學工程學研究所 |
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