https://scholars.lib.ntu.edu.tw/handle/123456789/465961
標題: | Dialysate lavage: An alternative solution for reducing the peritoneal implantation of poorly differentiated gastric cancer cells | 作者: | Liu S.-Y. Jeng J.-Y. Lin H.-C. KUAN-YU HUNG I-RUE LAI |
公開日期: | 2015 | 出版社: | S. Karger AG | 卷: | 55 | 期: | 1-2 | 起(迄)頁: | 24-34 | 來源出版物: | European Surgical Research | 摘要: | Background: Peritoneal lavage after cancer surgery is performed to reduce microscopic residual tumors in the peritoneum. This study evaluated the effects and mechanism of dialysate lavage in reducing the peritoneal implantation of gastric cancer cells. Methods: Gastric cancer cells (MKN45 or AGS) were cultured with 1.5% peritoneal dialysate (PD) or normal saline (NS) for 30 min. The in vitro cell susceptibility to dialysate, including cell proliferation, cell death, cleaved PARP expression, and mitochondrial membrane potential, was evaluated. A murine model for gastric cancer cell peritoneal seeding was established to test the effects of PD and NS lavage on animal survival and tumor growth. Results: A significant decrease in cell proliferation in PD and NS (75.2 ± 0.1 vs. 12.4 ± 0.2% in MKN45, p = 0.009; 58.2 ± 0.01 vs. 28.0 ± 0.01% in AGS, p = 0.008), an increase in mitochondrial permeability transition (93.0 ± 2.6 vs. 18.0 ± 2.9% in MKN45, p = 0.021; 86.8 ± 4.6 vs. 47.7 ± 10.2% in AGS, p < 0.001), and an increase in the expression of cleaved PARP and increased death (25.6 ± 9.4 vs. 16.9 ± 5.3% in MKN45, p = 0.031; 39.5 ± 5.1 vs. 20.9 ± 3.9% in AGS, p = 0.008) were recorded for gastric cancer cells separately exposed to PD and NS. Twenty-four days after inoculating MKN45 cells (5 × 106/0.1 ml) in the peritoneal cavity, the average number of seeded tumors was 67.3 ± 10.8, 92.3 ± 6.0, and 29.2 ± 16.7 (p = 0.032), and the total weight of tumors was 0.98 ± 0.21, 0.58 ± 0.12, and 0.31 ± 0.17 g (p = 0.008), respectively, for mice receiving sham operation, NS lavage, and PD lavage. The 45-day survival rate for the PD lavage group was 22% compared to 0% for the sham injection and NS lavage groups (p = 0.034). Conclusion: PD induced significant cytotoxicity in gastric cancer cells that was related to mitochondrial perturbation. The use of PD lavage was effective in reducing the peritoneal implantation of gastric cancers in a murine model. ? 2015 S. Karger AG, Basel. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924703484&doi=10.1159%2f000371345&partnerID=40&md5=50f3fe269d330f95ac0505a9409093d9 https://scholars.lib.ntu.edu.tw/handle/123456789/465961 |
ISSN: | 0014-312X | DOI: | 10.1159/000371345 | SDG/關鍵字: | nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; sodium chloride; cancer cell; cancer surgery; cell death; cell differentiation; cell proliferation; cell survival; Conference Paper; controlled study; cytotoxicity; dialysate; human; human cell; in vitro study; lymph node dissection; mitochondrial membrane potential; mitochondrial permeability; outcome assessment; peritoneal cavity; peritoneum; peritoneum lavage; priority journal; stomach cancer; stomach lavage; tumor growth; adenocarcinoma; animal; Bagg albino mouse; male; Neoplasms, Experimental; nude mouse; pathology; Peritoneal Neoplasms; peritoneum lavage; stomach tumor; tumor cell line; tumor seeding; Adenocarcinoma; Animals; Cell Line, Tumor; Humans; Male; Membrane Potential, Mitochondrial; Mice, Inbred BALB C; Mice, Nude; Neoplasm Seeding; Neoplasms, Experimental; Peritoneal Lavage; Peritoneal Neoplasms; Peritoneum; Stomach Neoplasms |
顯示於: | 解剖學暨細胞生物學科所 |
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