In vitro and in vivo wound healing-promoting activities of β-lapachone
Journal
American Journal of Physiology - Cell Physiology
Journal Volume
295
Journal Issue
4
Pages
C931-C943
Date Issued
2008
Author(s)
Abstract
Impaired wound healing is a serious problem for diabetic patients. Wound healing is a complex process that requires the cooperation of many cell types, including keratinocytes, fibroblasts, endothelial cells, and macrophages. β-Lapachone, a natural compound extracted from the bark of the lapacho tree (Tabebuia avellanedae), is well known for its antitumor, antiinflammatory, and antineoplastic effects at different concentrations and conditions, but its effects on wound healing have not been studied. The purpose of the present study was to investigate the effects of β-lapachone on wound healing and its underlying mechanism. In the present study, we demonstrated that a low dose of β-lapachone enhanced the proliferation in several cells, facilitated the migration of mouse 3T3 fibroblasts and human endothelial EAhy926 cells through different MAPK signaling pathways, and accelerated scrape-wound healing in vitro. Application of ointment with or without β-lapachone to a punched wound in normal and diabetic (db/db) mice showed that the healing process was faster in β-lapachone-treated animals than in those treated with vehicle only. In addition, β-lapachone induced macrophages to release VEGF and EGF, which are beneficial for growth of many cells. Our results showed that β-lapachone can increase cell proliferation, including keratinocytes, fibroblasts, and endothelial cells, and migration of fibroblasts and endothelial cells and thus accelerate wound healing. Therefore, we suggest that β-lapachone may have potential for therapeutic use for wound healing. Copyright ? 2008 the American Physiological Society.
SDGs
Other Subjects
4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole; anthra[1,9 cd]pyrazol 6(2h) one; beta lapachone; epidermal growth factor; epidermal growth factor receptor; mitogen activated protein kinase; mitogen activated protein kinase 14; vasculotropin; animal cell; animal experiment; animal model; article; cell growth; cell migration; cell proliferation; cell strain 3T3; controlled study; diabetes mellitus; drug effect; endothelium cell; fibroblast; human; human cell; in vitro study; in vivo study; keratinocyte; low drug dose; macrophage; mouse; nonhuman; priority journal; signal transduction; tissue regeneration; wound; wound healing; Animals; Anti-Infective Agents; Cell Cycle; Cell Line; Cell Movement; Cell Proliferation; Cells, Cultured; Humans; Male; Mice; Mice, Inbred C57BL; Mice, Inbred NOD; Mitogen-Activated Protein Kinase Kinases; Naphthoquinones; Skin; Wound Healing; Animalia; Mus; Tabebuia avellanedae
Type
journal article