Synthesis of water-based cationic polyurethane for antibacterial and gene delivery applications
Journal
Colloids and Surfaces B: Biointerfaces
Journal Volume
146
Pages
825-832
Date Issued
2016
Author(s)
Abstract
Cationic polymers are often used as antimicrobial materials and transfection reagents. Water-based process could reduce environmental pollution and prevent the risk of solvent residue in the final product. In this study, waterborne biodegradable cationic polyurethane (WCPU) was synthesized by reacting polycaprolactone (PCL diol), isophorone diisocyanate (IPDI), and N-methyldiethanolamine (N-MDEA) under 75°C. An aqueous dispersion of WCPU nanoparticles (NPs) could be acquired by vigorous stirring under acidic condition. The particles in the dispersion had an average size of ∼80 nm and a zeta potential of ∼60 mV. When cast into films, the contact angle of the film was ∼67° and the zeta potential was ∼16 mV. WCPU NPs demonstrated excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) (100% inhibition with a contact time of 3 h). Meanwhile, the antibacterial ratio of WCPU films to E. coli and S. aureus reached 100% after 24 h of contact. Moreover, WCPU NPs could be used as a transfection reagent without significant toxicity for concentrations less than 1000 μg/mL and showed the ability to condensate plasmid DNA. The transfection efficiency for HEK293T cells and hBMSCs was ∼60% and ∼30% at 48 h, respectively, after the transfection. Therefore, the WCPU synthesized in this study has potential antibacterial and gene delivery applications. © 2016 Elsevier B.V.
Subjects
Antibacterial; Gene delivery; Nanoparticle; Polyurethane; Transfection; Waterborne
SDGs
Other Subjects
Bacteria; Dispersions; Escherichia coli; Gene transfer; Genes; Molecular biology; Nanoparticles; Polycaprolactone; Polyurethanes; Zeta potential; Antibacterial; Antimicrobial materials; Environmental pollutions; Escherichia coli (E. coli); Gene Delivery; Isophorone diisocyanate; Transfection; Waterborne; Water pollution; antiinfective agent; cation; cyanic acid derivative; ethanolamine derivative; isophorone diisocyanate; n methyldiethanolamine; nanoparticle; plasmid DNA; polycaprolactone; polyurethan; reagent; unclassified drug; water; water based cationic polyurethane; antiinfective agent; cation; polyester; polymer; polyurethan; water; acidity; antibacterial activity; aqueous solution; Article; biodegradability; chemical procedures; chemical reaction; contact angle; controlled study; dispersion; drug delivery system; Escherichia coli; film; gene delivery system; genetic transfection; HEK293 cell line; HEK293T cell; hematopoietic stem cell; human; human cell; nonhuman; particle size; priority journal; Staphylococcus aureus; synthesis; toxic concentration; zeta potential; administration and dosage; cell survival; chemistry; drug effects; gene transfer; plasmid; Anti-Bacterial Agents; Cations; Cell Survival; Escherichia coli; Gene Transfer Techniques; HEK293 Cells; Humans; Plasmids; Polyesters; Polymers; Polyurethanes; Staphylococcus aureus; Water
Type
journal article