Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Medicine / 醫學院
  3. School of Medicine / 醫學系
  4. Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma
 
  • Details

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma

Journal
Cancer Science
Journal Volume
109
Journal Issue
11
Pages
3494-3502
Date Issued
2018
Author(s)
Lin L.-L.
Huang C.-C.
Wu M.-T.
WEN-MING HSU  
Chuang J.-H.
DOI
10.1111/cas.13790
URI
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054486546&doi=10.1111%2fcas.13790&partnerID=40&md5=2f8e28d3679a686767a4ba1dc08a33bc
https://scholars.lib.ntu.edu.tw/handle/123456789/469967
Abstract
The innate immune receptors, such as toll-like receptor 3 (TLR3), melanoma differentiation-associated 5 (MDA5) and retinoic acid-inducible gene-I (RIG-I), have been shown to be differentially expressed in neuroblastoma (NB) and promote dsRNA poly (I:C)-induced NB suppression in?vitro and in?vivo. However, the role of another important innate immune cytosolic sensor, laboratory of genetics and physiology 2 (LGP2), in the cancer behavior of NB remains unclear. Here, we demonstrated that the expression levels of LGP2 were either low or undetectable in all NB cell lines tested with or without MYCN amplification. LGP2 expression levels were significantly increased only in NB cells without MYCN amplification, including SK-N-AS and SK-N-FI after poly (I:C) treatment in?vitro and in mouse xenograft models. Ectopic expression of LGP2 in NB cells significantly enhanced poly (I:C)-induced NB cell death associated with downregulation of MDA5, RIG-I, MAVS and Bcl-2, as well as upregulation of Noxa and tBid. By immunofluorescence analyses, LGP2 localized mainly in the cytoplasm of NB cells after poly (I:C) treatment. In human NB tissue samples, cytoplasmic LGP2 expression was positively correlated with histological differentiation and inversely correlated with MYCN amplification. Positive cytoplasmic LGP2 expression in tumor tissues could predict a favorable outcome in NB patients independent of other prognostic factors. In short, LGP2 was effective in promoting poly (I:C)-induced NB suppression and cytoplasmic LGP2 can serve as an independent favorable prognostic factor in NB patients. ? 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
Subjects
LGP2; MDA5; neuroblastoma; polyinosinic-polycytidylic acid; RIG-I
SDGs

[SDGs]SDG3

Other Subjects
interferon induced helicase C domain containing protein 1; laboratory of genetic and physiology 2 protein; mitochondrial antiviral signaling protein; MYCN protein; protein bcl 2; protein Bid; protein Noxa; retinoic acid inducible protein I; tumor marker; unclassified drug; DHX58 protein, human; polyinosinic polycytidylic acid; RNA helicase; animal experiment; animal model; Article; cancer inhibition; cancer prognosis; child; controlled study; cytoplasm; down regulation; ectopic expression; female; gene amplification; human; human tissue; in vitro study; major clinical study; male; mouse; neuroblastoma; neuroblastoma cell line; nonhuman; priority journal; protein expression; protein localization; tissue differentiation; tumor xenograft; animal; cancer transplantation; cell cycle; cell proliferation; cell survival; down regulation; drug effect; genetics; infant; innate immunity; metabolism; neuroblastoma; preschool child; prognosis; tumor cell line; Animals; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Cell Survival; Child, Preschool; Cytoplasm; Down-Regulation; Female; Humans; Immunity, Innate; Infant; Male; Mice; Neoplasm Transplantation; Neuroblastoma; Poly I-C; Prognosis; RNA Helicases
Publisher
Blackwell Publishing Ltd
Type
journal article

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science