|Title:||Aberrant expression of annexin A10 is closely related to gastric phenotype in serrated pathway to colorectal carcinoma||Authors:||JIA-HUEI TSAI
|Issue Date:||2015||Publisher:||Nature Publishing Group||Journal Volume:||28||Journal Issue:||2||Start page/Pages:||268-278||Source:||Modern Pathology||Abstract:||
Annexin A10 (ANXA10) is a member of the ANX family that is normally expressed in gastric mucosa. ANXA10 was recently observed to be upregulated in sessile serrated adenoma, a precursor to microsatellite-unstable colorectal cancer. We investigated the use of ANXA10 in diagnosing colorectal carcinoma. In an immunohistochemical analysis, the intensity and quantity of ANXA10, MUC5AC, MUC6 and CDX2 in 123 colorectal carcinomas were graded. We determined the molecular status of BRAF and KRAS mutations, as well as the microsatellite instability status and the CpG island methylator phenotype in all colorectal carcinomas, and subcategorized into four molecular subgroups according to the molecular derangements. Nuclear ANXA10 staining was present in 36 colorectal carcinomas, exhibiting a strong significant association with the BRAF mutation status (P<0.0001) and positive CpG island methylator phenotype (P<0.0001), and a borderline significant association with high levels of microsatellite instability (P=0.072). The ANXA10-positive colorectal carcinomas were frequently positive for MUC5AC and MUC6, and were associated with absent or reduced CDX2 expression (all P<0.0001). According to a classification and regression tree analysis, ANXA10 is a superior marker for the molecular subtyping of colorectal carcinomas and represents a specific marker for colorectal cancers of the serrated pathway. Our results indicated that ANXA10 expression is implicated in gastric programming in serrated-pathway-associated colorectal carcinoma. ANXA10-positive colorectal carcinoma is highly associated with the molecular features of the serrated neoplasia pathway. ? 2015 USCAP, Inc All rights reserved.
|ISSN:||0893-3952||DOI:||10.1038/modpathol.2014.96||SDG/Keyword:||annexin; B Raf kinase; K ras protein; mucin; mucin 5AC; mucin 6ac; transcription factor Cdx2; unclassified drug; annexin; ANXA10 protein, human; tumor marker; Article; colorectal carcinoma; controlled study; CpG island; gene mutation; human; human tissue; immunohistochemistry; microsatellite instability; phenotype; priority journal; protein expression; stomach mucosa; adenocarcinoma; biosynthesis; cell transformation; colorectal tumor; DNA methylation; genetics; metabolism; nucleotide sequence; pathology; precancer; reverse transcription polymerase chain reaction; stomach; Adenocarcinoma; Annexins; Cell Transformation, Neoplastic; Colorectal Neoplasms; CpG Islands; DNA Methylation; DNA Mutational Analysis; Humans; Immunohistochemistry; Microsatellite Instability; Phenotype; Precancerous Conditions; Reverse Transcriptase Polymerase Chain Reaction; Stomach; Tumor Markers, Biological
|Appears in Collections:||病理學科所|
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