https://scholars.lib.ntu.edu.tw/handle/123456789/470934
標題: | Clearance kinetics of biomaterials affects stem cell retention and therapeutic efficacy | 作者: | Lai C.Y. Wu P.J. Roffler S.R. Lee S.T. Hwang S.M. SHOEI-SHEN WANG Wang K. Hsieh P.C.H. |
公開日期: | 2014 | 卷: | 15 | 期: | 2 | 起(迄)頁: | 564-573 | 來源出版物: | Biomacromolecules | 摘要: | The use of biomaterial carriers to improve the therapeutic efficacy of stem cells is known to augment cell delivery, retention, and viability. However, the way that carrier clearance kinetics boosts stem cell delivery and impacts stem cell function remains poorly characterized. In this study, we designed a platform to simultaneously quantify carrier clearance and stem cell retention to evaluate the impact of carrier clearance kinetics on stem cell retention. Additionally, a murine model of hindlimb ischemia was employed to investigate the effects of various cell retention profiles on mitigating peripheral arterial disease. To image the in vivo behaviors of material and cells, we used biotinylated hyaluronan with fluorescently labeled streptavidin and Discosoma sp. Red (Ds-Red)-expressing human mesenchymal stem cells. We found that the retention of transplanted stem cells was closely related to the remaining biomaterial. Furthermore, therapeutic effectiveness was also affected by stem cell retention. These results demonstrate that low-molecularweight hyaluronan had a slow clearance and high cell retention profile, improving the therapeutic efficacy of human stem cells. ? 2014 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896747668&doi=10.1021%2fbm401583b&partnerID=40&md5=e4257537aea44512d69cddd1b329b343 https://scholars.lib.ntu.edu.tw/handle/123456789/470934 |
ISSN: | 1525-7797 | DOI: | 10.1021/bm401583b | SDG/關鍵字: | Cell delivery; Cell functions; Cell retention; Human mesenchymal stem cells; Murine model; Peripheral arterial disease; Streptavidin; Therapeutic efficacy; Biological materials; Biomaterials; Cytology; Hyaluronic acid; Kinetics; Materials properties; Proteins; Stem cells; biomaterial; hyaluronic acid; streptavidin; biomaterial; animal experiment; animal model; article; biotinylation; clearance; controlled study; hindlimb; human; human cell; kinetics; limb ischemia; male; mesenchymal stem cell; mouse; nonhuman; peripheral occlusive artery disease; priority journal; stem cell; stem cell transplantation; animal; cell culture; chemistry; drug effects; ischemia; mesenchymal stem cell transplantation; mesenchymal stroma cell; nude mouse; Animals; Biocompatible Materials; Cells, Cultured; Hindlimb; Humans; Hyaluronic Acid; Ischemia; Kinetics; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells; Mice; Mice, Nude |
顯示於: | 醫學系 |
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