https://scholars.lib.ntu.edu.tw/handle/123456789/473279
標題: | Complementary roles of β-catenin and glutamine synthetase immunostaining in diagnosis of chemotherapy-treated and untreated hepatoblastoma | 作者: | Huang W.-J. JIA-HUEI TSAI YUNG-MING JENG |
公開日期: | 2017 | 出版社: | Elsevier B.V. | 卷: | 116 | 期: | 7 | 起(迄)頁: | 549-553 | 來源出版物: | Journal of the Formosan Medical Association | 摘要: | Background/Purpose This study aimed to evaluate the expression of β-catenin and its downstream target glutamine synthetase (GS) in hepatoblastoma (HB), and to evaluate the use of these two markers for diagnosing HB. Methods Eighteen untreated HBs and 22 HBs resected after neoadjuvant chemotherapy were analyzed using β-catenin and GS immunostaining. Results We detected nuclear β-catenin immunostaining in nearly all untreated HBs, including in fetal and embryonal epithelial components and in mesenchymal elements. We also observed diffuse GS expression in the epithelial component; however, it was frequently absent in embryonal and mesenchymal areas. In HBs resected after neoadjuvant chemotherapy, we recognized four histological patterns: fetal, hepatocellular-carcinoma-like, clear-cell, and normal-liver-like. All these patterns displayed diffuse GS expression. Fetal pattern showed diffuse nuclear β-catenin immunostaining. Nuclear β-catenin immunostaining was weak in the hepatocellular-carcinoma-like and clear-cell patterns. In normal-liver-like area, β-catenin expression was only located in the cell membrane. Conclusion The results suggest that nuclear β-catenin expression and diffuse GS immunostaining are the hallmarks of HB. Although epithelial and mesenchymal components of HB display nuclear β-catenin staining, this expression is attenuated following chemotherapy-induced cell maturation. GS immunostaining is especially useful for the assessment of section margins after neoadjuvant chemotherapy. ? 2016 |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006054763&doi=10.1016%2fj.jfma.2016.09.013&partnerID=40&md5=70fb7def0c3072ccdfc3b9c178b7fa42 https://scholars.lib.ntu.edu.tw/handle/123456789/473279 |
ISSN: | 0929-6646 | DOI: | 10.1016/j.jfma.2016.09.013 | SDG/關鍵字: | beta catenin; glutamate ammonia ligase; tumor marker; beta catenin; CTNNB1 protein, human; glutamate ammonia ligase; tumor marker; Article; cancer diagnosis; cell maturation; cell membrane; clinical article; controlled study; epithelium; hepatoblastoma; histopathology; human; human tissue; immunohistochemistry; liver cell carcinoma; mesenchyme; neoadjuvant chemotherapy; protein expression; protein function; hepatoblastoma; immunohistochemistry; liver tumor; beta Catenin; Biomarkers, Tumor; Glutamate-Ammonia Ligase; Hepatoblastoma; Humans; Immunohistochemistry; Liver Neoplasms |
顯示於: | 病理學科所 |
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