https://scholars.lib.ntu.edu.tw/handle/123456789/473919
標題: | Frequent allelic deletion at the FHIT locus associated with p53 overexpression in squamous cell carcinoma subtype of Taiwanese non-small-cell lung cancers | 作者: | Lee Y.-C. CHEN-TU WU JIN-YUAN SHIH Jou Y.-S. YIH-LEONG CHANG |
公開日期: | 2004 | 出版社: | Nature Publishing Group | 卷: | 90 | 期: | 12 | 起(迄)頁: | 2378-2383 | 來源出版物: | British Journal of Cancer | 摘要: | The fragile histidine triad (FHIT) gene, encompassing the FRA3B fragile site at chromosome 3p14.2, is a tumour suppressor gene involved in different tumour types including non-small-cell lung cancers (NSCLCs). In the current study, we examined for allelic deletion at the FHIT locus in 58 primary and microdissected NSCLCs, for which a clinicopathologic profile was available. We found a loss of 87.7% in heterozygosity (LOH) frequency at one or more microsatellite markers (D3S1289, D3S2408, D3S1766, D3S1312, D3S1600). Allelic deletion of D3S1766 was related to tumour histology in 10 of 11 squamous cell carcinomas (90.9%) displaying LOH compared with nine of 17 adenocarcinomas (52.9%; P = 0.049). Besides, in the subset of adenocarcinomas, a higher rate of LOH at D3S1289 was observed in male (six out of eight, 75%) than in female patients (four out of 17, 23.5%; P = 0.028). However, FHIT LOH was not correlated overall with a variety of clinical parameters including sex, smoking status, staging, lymph node metastasis and survival. These results indicated that the high frequency of FHIT gene disruption was important in the development of both squamous cell carcinomas and adenocarcinomas. Furthermore, there was no association between LOH at FHIT and protein expression, suggesting the presence of complex mechanisms of Fhit inactivation. On the other hand, the association between FHIT LOH and p53 protein overexpression assessment reached statistical significance (P = 0.026), implying that common alterations affect the two genes in tumour progression. ? 2004 Cancer Research UK. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-3042743715&doi=10.1038%2fsj.bjc.6601778&partnerID=40&md5=2b8f99952ed487a74e19784096492cb6 https://scholars.lib.ntu.edu.tw/handle/123456789/473919 |
ISSN: | 0007-0920 | DOI: | 10.1038/sj.bjc.6601778 | SDG/關鍵字: | fragile histidine triad protein; protein p53; acid anhydride hydrolase; fragile histidine triad protein; microsatellite DNA; protein p53; tumor protein; adenocarcinoma; adult; aged; article; cancer staging; cancer survival; controlled study; correlation analysis; female; gene deletion; gene disruption; gene expression; gene frequency; gene overexpression; heterozygosity loss; histology; human; human tissue; lung non small cell cancer; lung squamous cell carcinoma; lymph node metastasis; major clinical study; male; microdissection; microsatellite marker; priority journal; protein expression; sex difference; smoking; statistical significance; tumor growth; allele; biosynthesis; ethnology; gene expression profiling; genetics; heterozygosity loss; lung tumor; middle aged; pathology; Taiwan; upregulation; Acid Anhydride Hydrolases; Adult; Aged; Aged, 80 and over; Alleles; Carcinoma, Non-Small-Cell Lung; Female; Gene Expression Profiling; Humans; Loss of Heterozygosity; Lung Neoplasms; Male; Microsatellite Repeats; Middle Aged; Neoplasm Proteins; Sex Factors; Taiwan; Tumor Suppressor Protein p53; Up-Regulation |
顯示於: | 病理學科所 |
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