|Title:||Chitosan-assisted differentiation of porcine adipose tissue-derived stem cells into glucose-responsive insulin-secreting clusters||Authors:||Liu, H.-Y.
|Issue Date:||2017||Journal Volume:||12||Journal Issue:||3||Source:||PLoS ONE||Abstract:||
The unique advantage of easy access and abundance make the adipose-derived stem cells (ADSCs) a promising system of multipotent cells for transplantation and regenerative medicine. Among the available sources, porcine ADSCs (pADSCs) deserve especial attention due to the close resemblance of human and porcine physiology, as well as for the upcoming availability of humanized porcine models. Here, we report on the isolation and conversion of pADSCs into glucose-responsive insulin-secreting cells. We used the stromal-vascular fraction of the dorsal subcutaneous adipose from 9-day-old male piglets to isolate pADSCs, and subjected the cells to an induction scheme for differentiation on chitosan-coated plates. This one-step procedure promoted differentiation of pADSCs into pancreatic islet-like clusters (PILC) that are characterized by the expression of a repertoire of pancreatic proteins, including pancreatic and duodenal homeobox (Pdx-1), insulin gene enhancer protein (ISL-1) and insulin. Upon glucose challenge, these PILC secreted high amounts of insulin in a dosedependent manner. Our data also suggest that chitosan plays roles not only to enhance the differentiation potential of pADSCs, but also to increase the glucose responsiveness of PILCs. Our novel approach is, therefore, of great potential for transplantation-based amelioration of type 1 diabetes. ? 2017 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
|DOI:||10.1371/journal.pone.0172922||SDG/Keyword:||beta1 integrin; CD31 antigen; CD45 antigen; chitosan; glucose transporter 2; Hermes antigen; insulin; insulin gene enhancer protein; major histocompatibility antigen class 1; major histocompatibility antigen class 2; pancreas polypeptide; transcription factor PAX4; transcription factor PDX 1; unclassified drug; chitosan; culture medium; insulin; adipocyte; adipose derived stem cell; animal cell; antigen expression; Article; cell differentiation; chondrocyte; controlled study; gene expression; insulin release; male; nonhuman; osteocyte; pancreas islet beta cell; pig; protein expression; protein synthesis; adipose tissue; animal; cell differentiation; culture medium; cytology; drug effects; enzyme linked immunosorbent assay; secretion (process); stem cell; subcutaneous fat; Adipose Tissue; Animals; Cell Differentiation; Chitosan; Culture Media; Enzyme-Linked Immunosorbent Assay; Insulin; Stem Cells; Subcutaneous Fat; Swine
|Appears in Collections:||動物科學技術學系|
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