https://scholars.lib.ntu.edu.tw/handle/123456789/474032
標題: | Modulation of glucose and lipid metabolism by porcine adiponectin receptor 1-transgenic mesenchymal stromal cells in diet-induced obesemice | 作者: | Lin, Y.Y. Chen, C.Y. Lin, Y. Chiu, Y.P. Chen, C.C. Liu, B.H. Mersmann, H.J. Wu, S.C. SHIH-TORNG DING CHING-YI CHEN SHINN-CHIH WU YUAN-YU LIN |
公開日期: | 2013 | 卷: | 15 | 期: | 8 | 起(迄)頁: | 971-978 | 來源出版物: | Cytotherapy | 摘要: | Background aims: Obesity and its associated diseases demand better therapeutic strategies. Regenerative medicine combined with gene therapy has emerged as a promising approach in various clinical applications. Adiponectin (ApN) and its receptors have been demonstrated to play beneficial roles in modulating glucose and lipid homeostasis. In the current study, we tested such an approach by transplanting mesenchymal stromal cells (MSCs) from porcine ApN receptor (pAdipoR) 1-transgenic mice into high-fat/sucrose diet (HFSD)-fed mice. Methods: Twenty 6-week-old Friend virus B/NJNarl male mice were randomly assigned into four groups with the control fed a chow diet (chow) and others HFSD for 10 months. The HFSD groups were then intraperitoneally injected once per week for 8 weeks with placebo (200 μL phosphate-buffered saline), wild-type MSC (WT-MSC, 2× 106 cells/200 μL phosphate-buffered saline) or pAdipoR1-transgenic MSC (pR1-tMSC, 2× 106 cells/200 μL phosphate-buffered saline), respectively. Body weights, blood samples, tissue histology, and gene expression and protein levels of metabolism-associated genes were analyzed. Results: Both WT-MSC and pR1-tMSC transplantations restored the messenger RNA expression of AdipoR1, with those of glucose transporter 4 and 5'-adenosine monophosphate-activated protein kinase catalytic subunit α-1 and protein levels of pyruvate kinase induced by pR1-tMSC in the muscles of HFSD-fed mice. In the liver, both WT-MSC and pR1-tMSC ameliorated HFSD-induced hepatosteatosis, with the gene expression of lipoprotein lipase and hormone-sensitive lipase upregulated by the latter. Lastly, pR1-tMSC transplantation reduced fatty acid synthase mRNA levels in the adipose tissues of HFSD-fed mice. Conclusions: This study demonstrates the modulatory actions of MSC and pR1-tMSC on genes associated with glucose and lipid metabolism and provides insights into its therapeutic application for obesity-associated metabolic complication. ? 2013 International Society for Cellular Therapy. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84893515531&doi=10.1016%2fj.jcyt.2013.03.008&partnerID=40&md5=b84a24a1beb7779ca77e95ef46fe7f51 https://scholars.lib.ntu.edu.tw/handle/123456789/474032 |
DOI: | 10.1016/j.jcyt.2013.03.008 | SDG/關鍵字: | adiponectin receptor 1; fat droplet; glucose; glucose transporter 4; lipid; messenger RNA; pyruvate kinase; adiponectin receptor; cholesterol esterase; fatty acid synthase; glucose; glucose blood level; glucose transporter 4; lipoprotein lipase; messenger RNA; pyruvate kinase; adiponectin receptor; adiponectin receptor 1; adipose tissue; animal cell; animal experiment; animal model; animal tissue; Article; blood level; blood sampling; body weight; cell transplantation; controlled study; gene expression; gene function; gene identification; glucose metabolism; histology; human; human cell; lipid metabolism; male; mesenchymal stroma cell; mesenchymal stromal cell transplantation; molecular dynamics; mouse; nonhuman; obesity; treatment response; weight gain; animal; biological therapy; biosynthesis; cytology; gene therapy; genetics; glucose blood level; liver; liver cell; mesenchymal stem cell transplantation; metabolism; muscle; obesity; swine; transgenic animal; article; Diet-induced obesity; glucose blood level; mesenchymal stroma cell; Sus; Adipose Tissue; Animals; Animals, Genetically Modified; Blood Glucose; Cell- and Tissue-Based Therapy; Fatty Acid Synthase, Type I; Genetic Therapy; Glucose; Glucose Transporter Type 4; Hepatocytes; Lipid Metabolism; Lipoprotein Lipase; Liver; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells; Mice; Muscles; Obesity; Pyruvate Kinase; Receptors, Adiponectin; RNA, Messenger; Sterol Esterase; Swine; adiponectin receptor 1; diet-induced obesity; gene therapy; mesenchymal stromal cells; Adipose Tissue; Animals; Animals, Genetically Modified; Blood Glucose; Cell- and Tissue-Based Therapy; Fatty Acid Synthase, Type I; Genetic Therapy; Glucose; Glucose Transporter Type 4; Hepatocytes; Lipid Metabolism; Lipoprotein Lipase; Liver; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells; Mice; Muscles; Obesity; Pyruvate Kinase; Receptors, Adiponectin; RNA, Messenger; Sterol Esterase; Swine |
顯示於: | 動物科學技術學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。