https://scholars.lib.ntu.edu.tw/handle/123456789/474093
標題: | Sustained release of adipose-derived stem cells by thermosensitive chitosan/gelatin hydrogel for therapeutic angiogenesis | 作者: | NAI-CHEN CHENG Lin W.-J. THAI-YEN LING Tai-Horng Young |
公開日期: | 2017 | 出版社: | Elsevier Ltd | 卷: | 51 | 起(迄)頁: | 258-267 | 來源出版物: | Acta Biomaterialia | 摘要: | Adipose-derived stem cells (ASCs) secrete several angiogenic growth factors and can be applied to treat ischemic tissue. However, transplantation of dissociated ASCs has frequently resulted in rapid cell death. Therefore, we aimed to develop a thermosensitive chitosan/gelatin hydrogel that is capable of ASC sustained release for therapeutic angiogenesis. By blending gelatin in the chitosan thermosensitive hydrogel, we significantly enhanced the viability of the encapsulated ASCs. During in vitro culturing, the gradual degradation of gelatin led to sustained release of ASCs from the chitosan/gelatin hydrogel. In vitro wound healing assays revealed significantly faster cell migration by co-culturing fibroblasts with ASCs encapsulated in chitosan/gelatin hydrogel compared to pure chitosan hydrogels. Additionally, significantly higher concentrations of vascular endothelial growth factor were found in the supernatant of ASC-encapsulated chitosan/gelatin hydrogels. Co-culturing SVEC4-10 endothelial cells with ASC-encapsulated chitosan/gelatin hydrogels resulted in significantly more tube-like structures, indicating the hydrogel's potential in promoting angiogenesis. Chick embryo chorioallantoic membrane assay and mice wound healing model showed significantly higher capillary density after applying ASC-encapsulated chitosan/gelatin hydrogel. Relative to ASC alone or ASC-encapsulated chitosan hydrogel, more ASCs were also found in the wound tissue on post-wounding day 5 after applying ASC-encapsulated chitosan/gelatin hydrogel. Therefore, chitosan/gelatin thermosensitive hydrogels not only maintain ASC survival, they also enable sustained release of ASCs for therapeutic angiogenesis applications, thereby exhibiting great clinical potential in treating ischemic diseases. Statement of Significance Adipose-derived stem cells (ASCs) exhibit great potential to treat ischemic diseases. However, poor delivery methods lead to low cellular survival or dispersal of cells from target sites. In this study, we developed a thermosensitive chitosan/gelatin hydrogel that not only enhances the viability of the encapsulated ASCs, the gradual degradation of gelatin also result in a more porous architecture, leading to sustained release of ASCs from the hydrogel. ASC-encapsulated hydrogel enhanced in vitro wound healing of fibroblasts and tube formation of endothelial cells. It also promoted in vivo angiogenesis in a chick embryo chorioallantoic membrane assay and a mice wound model. Therefore, chitosan/gelatin hydrogel represents an effective delivery system that allows for controlled release of viable ASCs for therapeutic angiogenesis. ? 2017 Acta Materialia Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85011396225&doi=10.1016%2fj.actbio.2017.01.060&partnerID=40&md5=d7890b7d41cc3cc282c78ab2a9ee5a3b https://scholars.lib.ntu.edu.tw/handle/123456789/474093 |
ISSN: | 1742-7061 | DOI: | 10.1016/j.actbio.2017.01.060 | SDG/關鍵字: | Assays; Blending; Cell culture; Cell death; Endothelial cells; Fibroblasts; Hydrogels; Mammals; Stem cells; Adipose derived stem cells; Angiogenesis; Chitosan gelatins; Gelatin; Gelatin hydrogels; In-vitro; Sustained release; Therapeutic angiogenesis; Thermo sensitive; Thermo-sensitive hydrogel; Chitosan; chitosan; gelatin; vasculotropin; chitosan; gelatin; hydrogel; adipose derived stem cell; angiogenesis; animal cell; animal experiment; animal model; Article; capillary density; cell encapsulation; cell migration; cell proliferation; cell survival; cell viability; chorioallantoic membrane assay; controlled study; endothelium cell; fibroblast; heat sensitivity; human; human cell; hydrogel; in vitro study; mouse; nonhuman; priority journal; wound healing; adipose tissue; adult; animal; cell differentiation; chemistry; chick embryo; cytology; female; hydrogel; male; metabolism; middle aged; procedures; stem cell; stem cell transplantation; xenograft; Adipose Tissue; Adult; Animals; Cell Differentiation; Chick Embryo; Chitosan; Female; Gelatin; Heterografts; Humans; Hydrogels; Male; Middle Aged; Neovascularization, Physiologic; Stem Cell Transplantation; Stem Cells |
顯示於: | 醫學系 |
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