Modulation of keratin in adhesion, proliferation, adipogenic, and osteogenic differentiation of porcine adipose-derived stem cells
Journal
Journal of Biomedical Materials Research - Part B Applied Biomaterials
Journal Volume
105
Journal Issue
1
Pages
180-192
Date Issued
2017
Author(s)
Abstract
Recently, keratin attracts tremendous interest because of its intrinsic ability to interact with different cells. It has the potential to serve as a controllable extracellular matrix protein that can be used to demonstrate cell mechanism and cell-matrix interaction. However, there have been relatively few studies on the effects of keratin on stem cells. In the present work, we study the effects of human keratin on porcine adipose-derived stem cells (pASCs) and a series of selective cell lines: 3T3 fibroblasts, Madin-Darby canine kidney (MDCK) cells, and MG63 osteoblasts. Relative to un-treated culture plate, our results showed that keratin coating substrates promote cell adhesion and proliferation to above cell lines. Keratin also improved pASCs adhesion, proliferation, and enhanced cell viability. Evaluation of genetic markers showed that adipogenic and osteogenic differentiations of pASCs can be successfully induced, thus demonstrating that keratin did not influence the stemness of pASCs. Furthermore, keratin improved adipogenic differentiations of pASCs in terms of up-regulations in lipoprotein lipase, peroxisome proliferator-activated receptor gamma, and CCAAT-enhancer-binding protein alpha. The osteogenic markers type I collagen, runt-related transcription factor 2, and vitamin D receptor were also upregulated when pASCs cultured on keratin substrates. Therefore, keratin can serve as a biological derived material for surface modification and scaffold fabrication for biomedical purpose. The combination of keratin with stem cells may be a potential candidate for tissue repair in the field of regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 180-192, 2017.
SDGs
Other Subjects
Adhesion; Biological materials; Cell adhesion; Cell culture; Cells; Cytology; Differentiation (calculus); Keratin; Osteoblasts; Proteins; Stem cells; Surface treatment; Tissue; Adipogenic differentiations; Adipose derived stem cells; Adipose stem cells; Enhancer binding proteins; Extracellular matrix protein; Madin-Darby canine kidney cells; Osteogenic differentiation; Peroxisome proliferator-activated receptor; Scaffolds (biology); CCAAT enhancer binding protein alpha; collagen type 1; glyceraldehyde 3 phosphate dehydrogenase; keratin; lipoprotein lipase; peroxisome proliferator activated receptor gamma; polystyrene; thiol group; transcription factor RUNX2; vitamin D receptor; keratin; adipogenesis; adipose derived stem cell; animal cell; Article; bone development; cell adhesion; cell differentiation; cell proliferation; cell viability; controlled study; fibroblast; genetic marker; human; nonhuman; osteoblast; osteosarcoma cell; pig; reverse transcription polymerase chain reaction; upregulation; 3T3 cell line; adipogenesis; animal; bone development; cell adhesion; cell differentiation; cell proliferation; chemistry; cytology; dog; drug effects; MDCK cell line; metabolism; mouse; stem cell; 3T3 Cells; Adipogenesis; Animals; Cell Adhesion; Cell Differentiation; Cell Proliferation; Dogs; Keratins; Madin Darby Canine Kidney Cells; Mice; Osteogenesis; Stem Cells; Swine
Publisher
John Wiley and Sons Inc.
Type
journal article