The Study of Collagen Fibers in Various Tissues with Picro-Sirius Polarization
Journal
臺灣獸醫學雜誌
Journal Volume
33
Journal Issue
2
Pages
81-87
Date Issued
2007
Author(s)
JIANN-GWU LEE
CHIUNG-HSIANG CHENG
FUN-IN WANG
PEN-HENG CHANG
MICHAEL RS LIU
JEOU-JONG SHYU
Abstract
By using picro-sirius red stain and polarization microscopy, the collagen fibers in coronary artery with atherosclerosis, brachial artery with intimal thickening, and the cartilage and bone, were studied. The collagen fiber showed pale pink to red color after stained with picro-sirius red. With polarization microscopy, the thin collagen fiber showed green to yellow color, and the thick collagen fiber showed yellow to orange and red color. When rotating the circular specimen loading stage, the same birefringence could be observed periodically. The distribution of thick collagen fiber and thin collagen fiber could be distinguished among the atherosclerotic lesion. The thick collagen fiber and thin collagen fiber were interwoven in the intimal thickening lesion. The chondroid matrix was mainly distributed with thin collagen fiber, and the bone matrix was mainly distributed with thick collagen fiber. This method is more sensitive than Masson's trichrome stain for the examination of collagen fiber, and can be applied for the detailed study of collagen fiber in normal tissue and diseased condition.
Type
journal article