https://scholars.lib.ntu.edu.tw/handle/123456789/474916
標題: | In vivo metabolic imaging of insulin with multiphoton fluorescence of human insulin-Au nanodots | 作者: | Liu C.-L. Liu T.-M. Hsieh T.-Y. Liu H.-W. Chen Y.-S. Tsai C.-K. Chen H.-C. Lin J.-W. RON-BIN HSU TZUNG-DAU WANG Chen C.-C. CHI-KUANG SUN PI-TAI CHOU |
公開日期: | 2013 | 卷: | 9 | 期: | 12 | 起(迄)頁: | 2103-2110 | 來源出版物: | Small | 摘要: | Functional human insulin-Au nanodots (NDs) are synthesized for the in vivo imaging of insulin metabolism. Benefiting from its efficient red to near infrared fluorescence, deep tissue subcellular uptake of insulin-Au NDs can be clearly resolved through a least-invasive harmonic generation and two-photon fluorescence (TPF) microscope. In vivo investigations on mice ear and ex vivo assays on human fat tissues conclude that cells with rich insulin receptors have higher uptake of administrated insulin. Interestingly, the insulin-Au NDs can even permeate into lipid droplets (LDs) of adipocytes. Using this newly discovered metabolic phenomenon of insulin, it is found that enlarged adipocytes in type II diabetes mice have higher adjacent/LD concentration contrast with small-sized ones in wild type mice. For human clinical samples, the epicardial adipocytes of patients with diabetes and coronary artery disease (CAD) also show elevated adjacent/LD concentration contrast. As a result, human insulin-Au nanodots provide a new approach to explore subcellular insulin metabolism in model animals or patients with metabolic or cardiovascular diseases. Copyright ? 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052488827&doi=10.1002%2fsmll.201201887&partnerID=40&md5=960496d38b4da19e90a46fc37d17b7a9 https://scholars.lib.ntu.edu.tw/handle/123456789/474916 |
ISSN: | 1613-6810 | DOI: | 10.1002/smll.201201887 | SDG/關鍵字: | Bio-imaging; Cardio-vascular disease; Coronary artery disease; Gold nanodots; Multiphoton fluorescence; Multiphoton imaging; Near-infrared fluorescence; Two photon fluorescence; Diseases; Fluorescence; Mammals; Medical nanotechnology; Metabolism; Physiology; Tissue; Insulin; Animalia; Mus; gold; insulin; metal nanoparticle; gold; insulin; metal nanoparticle; chemistry; human; multiphoton microscopy; nanomedicine; procedures; article; chemistry; methodology; multiphoton microscopy; Gold; Humans; Insulin; Metal Nanoparticles; Microscopy, Fluorescence, Multiphoton; Nanomedicine; Gold; Humans; Insulin; Metal Nanoparticles; Microscopy, Fluorescence, Multiphoton; Nanomedicine |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。