Suppression of miR-19b enhanced the cytotoxic effects of mTOR inhibitors in human neuroblastoma cells
Journal
Journal of Pediatric Surgery
Journal Volume
51
Journal Issue
11
Pages
1818-1825
Date Issued
2016
Author(s)
Abstract
Background Mammalian target of rapamycin (mTOR) inhibitors exert significant antitumor effects on several cancer cell types. In this study, we investigated the effects of mTOR inhibitors, in particular the regulation of the microRNA, in neuroblastoma cells. Methods AZD8055 (a new mTOR inhibitor)- or rapamycin-induced cytotoxic effects on neuroblastoma cells were studied. Western blotting was used to investigate the expression of various proteins in the mTOR pathway. MicroRNA precursors and antagomirs were transfected into cells to manipulate the expression of target microRNA. Results AZD8055 exerted stronger cytotoxic effects than rapamycin in neuroblastoma cells (p?
Subjects
AZD8055; miR-19b; mTOR; Neuroblastoma
SDGs
Other Subjects
antagomir; azd 8055; DNA nucleotidylexotransferase; microRNA; microRNA 19b; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; rapamycin; unclassified drug; (5-(2,4-bis((3S)-3-methylmorpholin-4-yl)pyrido(2,3-d)pyrimidin-7-yl)-2-methoxyphenyl)methanol; immunosuppressive agent; microRNA; MIRN19 microRNA, human; morpholine derivative; MTOR protein, human; rapamycin; RNA; target of rapamycin kinase; apoptosis; Article; cell viability; DNA fragmentation; drug cytotoxicity; gene overexpression; genetic transfection; human; human cell; neuroblastoma cell; priority journal; real time polymerase chain reaction; RNA extraction; signal transduction; TUNEL assay; Western blotting; antagonists and inhibitors; gene expression regulation; genetics; metabolism; neuroblastoma; pathology; tumor cell line; Blotting, Western; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Humans; Immunosuppressive Agents; MicroRNAs; Morpholines; Neuroblastoma; Real-Time Polymerase Chain Reaction; RNA, Neoplasm; Sirolimus; TOR Serine-Threonine Kinases
Publisher
W.B. Saunders
Type
journal article