Andrographolide induces apoptosis of C6 glioma cells via the ERK-p53-caspase 7-PARP pathway
Journal
BioMed Research International
Journal Volume
2014
Date Issued
2014
Author(s)
Wang, Seu-Mei
Syu, Jhih-Pu
Chen, Ying
Peng, Yu-Sen
Abstract
Background. Glioma is the most malignant tumor of the central nervous system. Efforts on the development of new chemotherapy are mandatory. Andrographolide (AND), a diterpenoid lactone isolated from the Andrographis paniculata, has been shown to have antitumor activities in several types of cancer cells. Whether AND can exert its antitumor activity in glioblastoma cells remains unknown. This study examined the anticancer effects of AND, both in vitro and in vivo. Methods. Cell apoptosis was assayed by flow cytometry and nuclear staining. The signaling pathway for AND was determined by western blotting. The effects of AND on tumor growth was evaluated in a mouse model. Results and Conclusion. In vitro, with application of specific inhibitors and siRNA, AND-induced apoptosis was proven through ROS-ERK-P53-caspase 7-PARP signaling pathway. In vivo, AND significantly retarded tumor growth and caused regression of well-formed tumors in vivo. Furthermore, AND did not induce apoptosis or activate ERK and p53 in primary cultured astrocyte cells, and it may serve as a potential therapeutic candidate for the treatment of glioma. ? 2014 Shih-Hung Yang et al.
SDGs
Other Subjects
andrographolide; caspase 7; mitogen activated protein kinase 1; mitogen activated protein kinase 3; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; protein p53; reactive oxygen metabolite; small interfering RNA; andrographolide; Casp7 protein, mouse; caspase 7; diterpene; mitogen activated protein kinase p38; protein p53; animal cell; animal experiment; animal model; antineoplastic activity; apoptosis; Article; astrocyte; cancer cell culture; cancer growth; cancer regression; controlled study; drug determination; glioma cell; in vitro study; in vivo study; mouse; nonhuman; rat; signal transduction; animal; apoptosis; biosynthesis; cell proliferation; cell survival; drug effects; genetics; glioma; human; metabolism; pathology; tumor cell line; Animals; Apoptosis; Caspase 7; Cell Line, Tumor; Cell Proliferation; Cell Survival; Diterpenes; Glioma; Humans; MAP Kinase Signaling System; Mice; p38 Mitogen-Activated Protein Kinases; RNA, Small Interfering; Tumor Suppressor Protein p53
Publisher
Hindawi Publishing Corporation
Type
journal article