https://scholars.lib.ntu.edu.tw/handle/123456789/477421
標題: | Macrophage infiltration induces gastric cancer invasiveness by activating the β-catenin pathway | 作者: | MING-HSUN WU Lee W.-J. Hua K.-T. Kuo M.-L. MING-TSAN LIN |
公開日期: | 2015 | 出版社: | Public Library of Science | 卷: | 10 | 期: | 7 | 來源出版物: | PLoS ONE | 摘要: | Background Despite evidence that activated macrophages act in an inflammatory microenvironment to promote gastric tumorigenesis via β-catenin signaling, the effects of β-catenin signaling on gastric cancer cell metastasis and the relationship of these cells with surrounding tumor associated macrophages have not been directly studied. Methods Immunohistochemical staining was employed to analyze 103 patients. An invasion assay was used to evaluate the relationship between macrophages and gastric cancer cells. β-catenin gain-of-function and loss-of-function approaches were performed. To assess the β-catenin regulation mechanism in gastric cancer cells, Western blotting and reverse-transcription polymerase chain reaction were used. Results Increased density of macrophages was associated with advanced stage and poor survival. Gastric cancer cell lines co-cultured with macrophages conditioned medium showed increased nuclear accumulation of β-catenin and increased invading ability. AKT but not ERK regulated β-catenin translocation. MMP7 and CD44, both β-catenin downstream genes, were involved in macrophage-activated gastric cancer cell invasion. Conclusion(s) Collectively, the clinical data suggest that macrophage infiltration is correlated with increased grade and poor prognosis for gastric cancer patients who underwent radical resection. Macrophages may induce invasiveness by activating the β-catenin pathway. ? 2015 Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84941963250&doi=10.1371%2fjournal.pone.0134122&partnerID=40&md5=e659d8b7436fdc6bb5103a5c2bb29b55 https://scholars.lib.ntu.edu.tw/handle/123456789/477421 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0134122 | SDG/關鍵字: | beta catenin; CD68 antigen; cyclin D1; Hermes antigen; matrilysin; mitogen activated protein kinase; protein kinase B; tumor necrosis factor alpha; Wnt protein; beta catenin; adult; Article; cancer prognosis; cancer staging; cancer survival; CD44 gene; cell density; controlled study; cyclin D1 gene; cytokine production; female; gain of function mutation; gastric cancer cell line; gene; gene expression regulation; human; human cell; human tissue; immunohistochemistry; intracellular signaling; loss of function mutation; macrophage activation; macrophage migration; major clinical study; male; metastasis potential; middle aged; predictive value; protein expression; protein function; protein transport; reverse transcription polymerase chain reaction; stomach cancer; survival prediction; tumor associated leukocyte; tumor invasion; tumor microenvironment; Western blotting; Wnt signaling pathway; aged; cell nucleus; immunology; macrophage; metabolism; pathology; stomach tumor; tumor cell line; Aged; beta Catenin; Cell Line, Tumor; Cell Nucleus; Female; Humans; Macrophage Activation; Macrophages; Male; Middle Aged; Neoplasm Invasiveness; Protein Transport; Stomach Neoplasms |
顯示於: | 醫學系 |
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