https://scholars.lib.ntu.edu.tw/handle/123456789/478422
標題: | Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis | 作者: | Tyan S.-W. WEN-HUNG KUO Huang C.-K. Pan C.-C. Shew J.-Y. KING-JEN CHANG Lee E.Y.-H.P. Lee W.-H. |
公開日期: | 2011 | 卷: | 6 | 期: | 1 | 起(迄)頁: | e15313 | 來源出版物: | PLoS ONE | 摘要: | It has been well documented that microenvironment consisting of stroma affects breast cancer progression. However, the mechanisms by which cancer cells and fibroblasts, the major cell type in stroma, interact with each other during tumor development remains to be elucidated. Here, we show that the human cancer-associated fibroblasts (CAFs) had higher activity in enhancing breast tumorigenecity compared to the normal tissue-associated fibroblasts (NAFs) isolated from the same patients. The expression level of hepatocyte growth factor (HGF) in these fibroblasts was positively correlated with their ability to enhance breast tumorigenesis in mice. Deprivation of HGF using a neutralizing antibody reduced CAFmediated colony formation of human breast cancer cells, indicating that CAFs enhanced cancer cell colony formation mainly through HGF secretion. Co-culture with human breast cancer MDA-MB-468 cells in a transwell system enhanced NAFs to secret HGF as well as promote tumorigenecity. The newly gained ability of these "educated" NAFs became irreversible after continuing this process till fourth passage. These results suggested that breast cancer cells could alter the nature of its surrounding fibroblasts to secrete HGF to support its own progression through paracrine signaling. ? 2011 Tyan et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79251586559&doi=10.1371%2fjournal.pone.0015313&partnerID=40&md5=f75d8c3e36bedaee102d7c4244b080f3 https://scholars.lib.ntu.edu.tw/handle/123456789/478422 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0015313 | SDG/關鍵字: | scatter factor; scatter factor; animal experiment; animal model; article; breast cancer; breast carcinogenesis; cancer cell culture; cancer growth; carcinogenicity; coculture; colony formation; controlled study; female; fibroblast; human; human cell; mouse; nonhuman; paracrine signaling; protein expression; protein secretion; animal; breast tumor; cell transformation; disease course; experimental neoplasm; metabolism; paracrine signaling; pathology; physiology; secretion; tumor cell line; tumor microenvironment; Mus; Animals; Breast Neoplasms; Cell Line, Tumor; Cell Transformation, Neoplastic; Coculture Techniques; Disease Progression; Female; Fibroblasts; Hepatocyte Growth Factor; Humans; Mammary Neoplasms, Animal; Mice; Paracrine Communication; Tumor Microenvironment |
顯示於: | 醫學系 |
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