Clinical and immunological responses to undiluted and diluted smallpox vaccine with vaccinia virus of Lister strain
Journal
Vaccine
Journal Volume
24
Journal Issue
4
Pages
510-515
Date Issued
2006
Author(s)
Abstract
The potential to increase the supply of vaccine by diluting the vaccinia virus of Lister strain to face possible bioterrorism with smallpox was evaluated. Vaccinia-na?ve subjects (n = 97) were randomized to receive either undiluted or diluted (1:5, 1:10) vaccine, and previously vaccinated subjects (n = 122) were randomized to receive either undiluted or diluted (1:10, 1:30) vaccine. Except two subjects who received 1:30 diluted vaccine, the vaccination of all subjects was successful clinically. All subjects had significant vaccinia-specific T cell and antibody responses. The diluted vaccine was not associated with decreased local or systemic reactions, lower T cell responses, or higher antibody titers when compared with undiluted vaccine. Here we show the diluted vaccine of Lister strain can be used in vaccinia-na?ve subjects and previously vaccinated subjects if viral titer ?108 and 107.5 pfu/mL after dilution, respectively. The reactogenicity of vaccinia virus may not be a dose-dependent response. ? 2005 Elsevier Ltd. All rights reserved.
SDGs
Other Subjects
antibody; smallpox vaccine; vaccinia vaccine; adult; aged; antibody response; antibody titer; article; cellular immunity; clinical trial; controlled clinical trial; controlled study; dilution; dose response; drug efficacy; drug fever; drug induced headache; fatigue; female; human; immune response; lymphadenopathy; male; myalgia; normal human; priority journal; randomized controlled trial; single blind procedure; smallpox; T lymphocyte; Vaccinia virus; virus strain; Adult; Antibodies, Viral; Dose-Response Relationship, Immunologic; Female; Humans; Male; Middle Aged; Neutralization Tests; Single-Blind Method; Smallpox Vaccine; T-Lymphocytes, Cytotoxic; Vaccination; Vaccinia virus; Variola virus
Type
journal article