|Title:||Aberrant p53 expression correlates with expression of vascular endothelial growth factor mRNA and interleukin-8 mRNA and neoangiogenesis in non-small-cell lung cancer||Authors:||ANG YUAN
|Issue Date:||2002||Journal Volume:||20||Journal Issue:||4||Start page/Pages:||900-910||Source:||Journal of Clinical Oncology||Abstract:||
Purpose: To evaluate interactions between expressions of tumor suppressor gene p53 and angiogenic factors vascular endothelial cell growth factor (VEGF) and interleukin-8 (IL-8) and their effect on tumor angiogenesis and patient prognosis in non-small-cell lung cancer (NSCLC). Patients and Methods: p53, VEGF, IL-8, and the microvessel endothelium were immunostained, and VEGF and IL-8 mRNA expression were quantified using the real-time quantitative reverse-transcription polymerase chain reaction in 65 NSCLC surgical specimens. Aberrant p53 expression was correlated with VEGF and IL-8 mRNA expression, microvessel count (MVC), other clinical-pathologic variables, and patients' survival. Results: Tumors with high aberrant p53 expression showed significantly higher VEGF and IL-8 mRNA expression and MVC than those with low aberrant p53 expression (P < .001). When tested as a continuous variable, aberrant p53 expression correlated strongly and positively with VEGF and IL-8 mRNA expression and MVC (P < .0001). Tumors with high aberrant p53 expression were associated with mediastinal or distant lymph node metastasis (P = .006). Survival and postoperative relapse time were significantly shorter in patients with high aberrant p53 expression tumors than in those with low aberrant expression tumors (P < .0001). A significant difference in survival was also seen between patients with high and low tumoral VEGF mRNA expression and between those with high and low tumoral IL-8 mRNA expression (P < .0001). Conclusion: We report here for the first time that aberrant p53 expression is strongly positively correlated with VEGF mRNA and IL-8 mRNA expression in NSCLC. This result indicates that aberrant p53 expression may play a significant role in regulation of VEGF and IL-8 expression and be involved in controlling angiogenesis and explains the adverse prognosis of cancers with high aberrant p53 expression. ? 2002 by American Society of Clinical Oncology.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/479338||ISSN:||0732-183X||DOI:||10.1200/JCO.20.4.900||SDG/Keyword:||interleukin 8; messenger RNA; protein p53; vasculotropin; adult; aged; angiogenesis; article; cancer inhibition; cancer survival; controlled study; disease association; endothelium; female; human; human tissue; immunohistochemistry; lung non small cell cancer; lymph node metastasis; major clinical study; male; mediastinum metastasis; microvasculature; priority journal; prognosis; protein expression; protein interaction; reverse transcription polymerase chain reaction; tumor suppressor gene; Adult; Aged; Carcinoma, Non-Small-Cell Lung; DNA, Neoplasm; Endothelial Growth Factors; Female; Gene Expression Regulation, Neoplastic; Genes, p53; Humans; Immunohistochemistry; Interleukin-8; Lung Neoplasms; Lymphokines; Male; Middle Aged; Neoplasm Invasiveness; Neoplasm Metastasis; Neovascularization, Pathologic; Prognosis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Survival Analysis; Tumor Suppressor Protein p53; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors
|Appears in Collections:||醫學院附設醫院 (臺大醫院)|
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