https://scholars.lib.ntu.edu.tw/handle/123456789/479662
標題: | Comparing the utilities of different multilocus sequence typing schemes for identifying outbreak strains of mycobacterium abscessus subsp. Massiliense | 作者: | ARISTINE CHENG HSIN-YUN SUN Tsai, Yi-Tzu SHU-YUAN CHANG UN-IN WU PO-REN HSUEH WANG-HUEI SHENG YEE-CHUN CHEN SHAN-CHWEN CHANG |
公開日期: | 2020 | 卷: | 58 | 期: | 1 | 起(迄)頁: | e01304-19 | 來源出版物: | Journal of Clinical Microbiology | 摘要: | Outbreaks of infections by Mycobacterium abscessus, particularly subspecies massiliense, are increasingly reported worldwide. Several multilocus sequence typing (MLST) protocols for grouping international outbreak strains have been developed but not yet directly compared. Using the three-gene (hsp65, rpoB, and secA1), seven-gene (argH, cya, glpK, gnd, murC, pta, and purH) and thirteen-gene (all of the preceding genes plus gdhA, pgm, and pknA) MLST schemes, we identified 22, 38, and 40 unique sequence types (STs), respectively, among a total of 139 nonduplicated M. abscessus isolates. Among subspecies massiliense, three-gene MLST not only clustered all outbreak strains together (in 100% agreement with the seven-gene and thirteen-gene schemes), but it also distinguished between two new STs that would have been grouped together by the seven-gene MLST but were distinct by the thirteen-gene MLST owing to differences in hsp65, rpoB, and pknA. Here, we show that an abbreviated MLST may be useful for simultaneous identification of M. abscessus the subspecies level and screening M. abscessus subsp. massiliense isolates with outbreak potential. ? 2019 American Society for Microbiology. All Rights Reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/479662 | ISSN: | 0095-1137 | DOI: | 10.1128/JCM.01304-19 | SDG/關鍵字: | allele; argH gene; Article; bacterial gene; bacterium isolate; comparative study; controlled study; cya gene; gdhA gene; gene locus; genetic conservation; genetic variability; glpK gene; gnd gene; hsp65 gene; multilocus sequence typing; murC gene; Mycobacterium massiliense; nonhuman; pgm gene; pknA gene; polymerase chain reaction; priority journal; pta gene; pulsed field gel electrophoresis; purH gene; repetitive unit sequence based PCR; rpoB gene; secA1 gene; single nucleotide polymorphism; atypical mycobacteriosis; DNA sequence; epidemic; genetics; human; microbiology; Mycobacterium abscessus; phylogeny; procedures; bacterial DNA; bacterial protein; Bacterial Proteins; Disease Outbreaks; DNA, Bacterial; Humans; Multilocus Sequence Typing; Mycobacterium abscessus; Mycobacterium Infections, Nontuberculous; Phylogeny; Sequence Analysis, DNA |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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