https://scholars.lib.ntu.edu.tw/handle/123456789/480858
標題: | The effect of the repression of oxidative stress on tenocyte differentiation: A preliminary study of a rat cell model using a novel differential tensile strain bioreactor | 作者: | MING-YEN HSIAO Lin, Ping Cheng Liao, Wei Hao WEN-SHIANG CHEN Hsu, Chia Hsien He, Cheng Kun Wu, Ya Wen Gefen, Amit Iafisco, Michele Liu, Lixin FENG-HUEI LIN |
關鍵字: | Cell model | Oxidative stress | Tendinopathy | 公開日期: | 2-七月-2019 | 出版社: | MDPI | 卷: | 20 | 期: | 14 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Because of limitations in the current understanding of the exact pathogenesis of tendinopathy, and the lack of an optimal experimental model, effective therapy for the disease is currently unavailable. This study aims to prove that repression of oxidative stress modulates the differentiation of tendon-derived cells (TDCs) sustaining excessive tensile strains, and proposes a novel bioreactor capable of applying differential tensile strains to cultured cells simultaneously. TDCs, including tendon-derived stem cells, tenoblasts, tenocytes, and fibroblasts, were isolated from the patellar tendons of Sprague-Dawley rats. Cyclic uniaxial stretching with 4% or 8% strain at 0.5 Hz for 8 h was applied to TDCs. TDCs subjected to 8% strain were treated with epigallocatechin gallate (EGCG), piracetam, or no medication. Genes representing non-tenocyte lineage (Pparg, Sox9, and Runx2) and type I and type III collagen were analyzed by quantitative polymerase chain reaction. The 8% strain group showed increased expression of non-tenocyte lineage genes and type III/type I collagen ratios compared with the control and 4% strain groups, and the increased expression was ameliorated with addition of EGCG and piracetam. The model developed in this work could be applied to future research on the pathophysiology of tendinopathy and development of treatment options for the disease. Repression of oxidative stress diminishes the expression of genes indicating aberrant differentiation in a rat cell model, which indicates potential therapeutic intervention of tendinopathy, the often relentlessly degenerate condition. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/480858 | ISSN: | 16616596 | DOI: | 10.3390/ijms20143437 |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。