|Title:||The effect of ephrin-A1 on resistance to Photofrin-mediated photodynamic therapy in esophageal squamous cell carcinoma cells||Authors:||Yang P.-W.
|Issue Date:||2015||Publisher:||Springer-Verlag London Ltd||Journal Volume:||30||Journal Issue:||9||Start page/Pages:||2353-2361||Source:||Lasers in Medical Science||Abstract:||
Esophageal squamous cell carcinoma (ESCC), the most prevalent cell type of esophageal cancer, remains a dismal disease with poor prognosis. Photodynamic therapy (PDT) is a minimally invasive treatment option for early esophageal cancer. To explore possible factors involved in resistance to PDT in esophageal cancer cells, we selected PDT-resistant subcell lines by repeated treatment of CE48T/VGH (CE48T) ESCC cells with Photofrin-PDT and then analyzed the global gene modulations in the PDT-resistant cells by whole-genome microarray. More than 700 genes reached a fold change greater than 1.5 in each of the PDT-resistant cells compared to parental cells. Among these genes, both tumor necrosis factor (TNF) and EFNA1 genes were significantly upregulated in resistant cell lines. However, they were significantly downregulated in Photofrin-PDT-treated cells compared to untreated cells. The observations made in the microarray analysis were further confirmed by quantitative PCR. We observed that recombinant tumor necrosis factor alpha (TNF-α) activated the gene expression of EFNA1 at both the messenger RNA (mRNA) level and the protein level in CE48T cells. Functional analysis showed that when incubated with oligomeric and monomeric ephrin-A1 simultaneously, ESCC cells became significantly resistant to Photofrin-PDT. Functional analysis further suggested that transmembrane and soluble ephrin-A1 may cooperate to enhance resistance to Photofrin-PDT in ESCC cells. ? 2015, Springer-Verlag London.
|ISSN:||0268-8921||DOI:||10.1007/s10103-015-1812-8||SDG/Keyword:||ephrin A1; ephrin receptor A2; messenger RNA; photofrin; protein; recombinant tumor necrosis factor alpha; tumor necrosis factor; ephrin A1; photofrin II; photosensitizing agent; Article; cancer cell; controlled study; esophageal squamous cell carcinoma; gene expression; human; human cell; in vitro study; microarray analysis; photodynamic therapy; priority journal; protein expression; upregulation; Carcinoma, Squamous Cell; down regulation; drug effects; drug resistance; Esophageal Neoplasms; genetics; pathology; photochemotherapy; radiation response; tumor cell line; Carcinoma, Squamous Cell; Cell Line, Tumor; Dihematoporphyrin Ether; Down-Regulation; Drug Resistance, Neoplasm; Ephrin-A1; Esophageal Neoplasms; Humans; Photochemotherapy; Photosensitizing Agents; Up-Regulation
|Appears in Collections:||醫學系|
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