|Title:||Molecular characterization of invasive subpopulations from an esophageal squamous cell carcinoma cell line||Authors:||Chen Y.-K.
|Keywords:||Esophageal cancer; Invasiveness; Matrix metalloproteinase-1; MMP-1; p-Src (pY416); TIMP-2; Tissue inhibitor of metalloproteinase-2; Transwell invasion chamber; Vimentin||Issue Date:||2010||Journal Volume:||30||Journal Issue:||3||Start page/Pages:||727-736||Source:||Anticancer Research||Abstract:||
Background: Once diagnosed, esophageal cancer has a very low overall 5-year survival rate. This study investigates the mechanisms behind the invasiveness and severity of esophageal squamous cell carcinoma (ESCC). Materials and Methods: Transwell invasion chamber was used to subdivide one Taiwanese ESCC cell line, CE81T/VGH, into sublines (CE81T-0, CE81T-1, CE81T-2, CE81T-3, and CE81T-4) in four rounds of assays; the most invasive were identified, and various factors related to their invasiveness measured. Results: CE81T-1, CE81T-2, CE81T-3 and CE81T-4 sublines were significantly more invasive than the parental cells (CE81T/VGH) and CE81T-0 subline. CE81T-1 and CE81T-4, the sublines we chose to study further, had significantly greater colony-forming ability (3.5- to 2.7-fold) and wound migrating activity (1.95- to 2.6-fold) than the parental cells in vitro (p<0.01). They also displayed greater tumorigenesis in immunodeficient BALB/c Foxlnn mice than the parental cells. We found an inverse correlation between expression of tissue inhibitor of metalloproteinase-2 and invasive ability, and a significant positive correlation between expressions of matrix metalloproteinase-1, vimentin, and p-Src (pY416) in these cell lines and their invasiveness (all p<0.05). Conclusion: The subline model may be used to study the molecular and genetic mechanisms underlying the invasion and metastasis of ESCC.
|ISSN:||0250-7005||SDG/Keyword:||gelatinase A; interstitial collagenase; steroid receptor coactivator 1; vimentin; animal experiment; animal model; article; cancer invasion; carcinogen testing; cell migration; cell motility; cell subpopulation; colony forming unit; controlled study; disease severity; enzyme activity; esophagus carcinoma; flow cytometry; human; human cell; in vitro study; in vivo study; metastasis; mouse; nonhuman; priority journal; protein expression; single nucleotide polymorphism; squamous cell carcinoma; tumor growth; Western blotting; wound healing; Animals; Blotting, Western; Carcinoma, Squamous Cell; Cell Growth Processes; Cell Line, Tumor; Cell Movement; Esophageal Neoplasms; Flow Cytometry; Humans; Male; Matrix Metalloproteinase 1; Matrix Metalloproteinase 2; Mice; Mice, Inbred BALB C; Mice, Nude; Middle Aged; Neoplasm Invasiveness; Neoplasm Metastasis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Tissue Inhibitor of Metalloproteinase-2; Vimentin
|Appears in Collections:||醫學系|
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