https://scholars.lib.ntu.edu.tw/handle/123456789/484268
標題: | Patho-finder-A fast and accurate program for pathogen identification through RNA-seq | 作者: | Wu, C.-T. Hsiao, T.-H. Chiu, Y.-C. Hsu, Y.-C. Chuang, E.Y. Chen, Y. ERIC YAO-YU CHUANG |
關鍵字: | next generation sequencing; pathogen; RNA-seq | 公開日期: | 2015 | 起(迄)頁: | 1148-1151 | 來源出版物: | Proceedings - 2015 IEEE International Conference on Bioinformatics and Biomedicine, BIBM 2015 | 摘要: | Technology of next generation sequencing to detect pathogens of sample can impact human health by revealing pathogens which cause disease. Several workflow has developed in purposed to detect pathogens in next generation sequencing data. However, the requirement of computation power of these workflows limited the application. The time consuming problem make the workflow difficult to detect datasets with large sample size. Here we presented Patho-finder, a fast and accurate workflow designed for detecting pathogen in RNA sequencing data. We have evaluated performance of Patho-finder by three aspects. First, we evaluate performance by alter the data features, to see how Patho-finder work under different simulation conditions. Next, we compare the time consuming and accuracy between Patho-finder and existing workflow. At last, we used Patho-finder on the RNA-seq of cell lines with known virus-infected. The validation result demonstrated our approach could finish the task in real datasets. ? 2015 IEEE. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/484268 | DOI: | 10.1109/BIBM.2015.7359843 | SDG/關鍵字: | Bioinformatics; Cell culture; RNA; Viruses; Computation power; Human health; Next-generation sequencing; Pathogen identification; Real data sets; Rna sequencing; Sample sizes; Validation results; Pathogens |
顯示於: | 生醫電子與資訊學研究所 |
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