Regulation of lipid droplets in live preadipocytes using optical diffraction tomography and Raman spectroscopy
Journal
Optics Express
Journal Volume
27
Journal Issue
16
Pages
22994-23008
Date Issued
2019
Author(s)
Hsieh, C.-M.
Liu, P.Y.
Chin, L.K.
Zhang, J.B.
Wang, K.
Ser, W.
Bourouina, T.
Leprince-Wang, Y.
Liu, A.-Q.
Abstract
Lipid droplets have gained strong interest in recent years to comprehend how they function and coordinate with other parts of the cell. However, it remains challenging to study the regulation of lipid droplets in live preadipocytes using conventional microscopic techniques. In this paper, we study the effects of fatty acid stimulation and cell starvation on lipid droplets using optical diffraction tomography and Raman spectroscopy by measuring size, refractive index, volume, dry mass and degree of unsaturation. The increase of fatty acids causes an increase in the number and dry mass of lipid droplets. During starvation, the number of lipid droplets increases drastically, which are released to mitochondria to release energy. Studying lipid droplets under different chemical stimulations could help us understand the regulation of lipid droplets for metabolic disorders, such as obesity and diabetes. © 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
SDGs
Other Subjects
Diffraction; Fatty acids; Optical systems; Raman spectroscopy; Refractive index; Tomography; Chemical stimulation; Degree of unsaturations; Dry mass; Lipid droplets; Metabolic disorders; Microscopic techniques; Optical diffraction tomography; Preadipocytes; Drops; fat droplet; 3T3-L1 cell line; adipocyte; animal; calibration; holography; metabolism; mouse; optical tomography; particle size; procedures; Raman spectrometry; time lapse imaging; 3T3-L1 Cells; Adipocytes; Animals; Calibration; Holography; Lipid Droplets; Mice; Particle Size; Spectrum Analysis, Raman; Time-Lapse Imaging; Tomography, Optical
Type
journal article