Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy.
Journal
33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2011, Boston, MA, USA, August 30 - Sept. 3, 2011
Pages
490-493
Date Issued
2011
Author(s)
Wei, Shih-Chung
Chuang, Tsung-Liang
Lu, Hui-Hsin
Chang, Chia-Chen
Wang, Da-Shin
Sung, Kung-Bin
Abstract
Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (100 nm) of an atomic force microscope (AFM) tip and performed localized LAMP reaction of hepatitis B virus (HBV). By using this tip-based reaction, the replicated HBV DNA can be directly imaged to be 400500 nm spots by using two-photon excitation fluorescence microscopy. ? 2011 IEEE.
SDGs
Other Subjects
Atomic force microscopes; Biological reaction; DNA replications; Hepatitis B virus; Nano-meter scale; Two photon microscopy; Two-photon excitation fluorescence microscopy; Atomic force microscopy; Fluorescence; Fluorescence microscopy; Photons; Viruses; Computer viruses; virus DNA; article; DNA probe; genetic procedures; genetics; Hepatitis B virus; image enhancement; methodology; molecular imaging; multiphoton microscopy; nucleic acid amplification; ultrastructure; DNA Probes; DNA, Viral; Hepatitis B virus; Image Enhancement; Microscopy, Fluorescence, Multiphoton; Molecular Imaging; Molecular Probe Techniques; Nucleic Acid Amplification Techniques
Type
conference paper