https://scholars.lib.ntu.edu.tw/handle/123456789/485619
Title: | Circular RNA TUBD1 Acts as the miR-146a-5p Sponge to Affect the Viability and Pro-Inflammatory Cytokine Production of LX-2 Cells through the TLR4 Pathway | Authors: | Niu H. Zhang L. Chen Y.-H. Yuan B.-Y. Wu Z.-F. CHIA-HSIEN CHENG Lin Q. Zeng Z.-C. |
Issue Date: | 2020 | Publisher: | Radiation Research Society | Journal Volume: | 193 | Journal Issue: | 4 | Start page/Pages: | 383-393 | Source: | Radiation Research | Abstract: | The functions and molecular mechanism of circRNAs in the development of radiation-induced liver disease (RILD) remain largely unknown. The goal of this study was to explore the expression and potential role of a new circular RNA, named circTUBD1, in irradiated and lipopolysaccharide (LPS)-stimulated human hepatic stellate cell (HSC) line LX-2 cells. The expression of circTUBD1 was significantly upregulated in irradiated and LPS-stimulated LX-2 cells compared to non-treated LX-2 cells. To explore the functions of circTUBD1, small interfering RNAs targeting circTUBD1 were designed. Silencing circTUBD1 inhibited proliferation, promoted apoptosis of LX-2 cells, and significantly decreased the expression level of pro-inflammatory cytokines, including IL-1β, IL-6 and TNF-α in irradiated and LPS-stimulated LX-2 cells. Mechanistic analysis suggested that circTUBD1 acted as the miR-146a-5p sponge to affect pro-inflammatory cytokine production through regulating expression of Toll-like receptor 4 (TLR4), interleukin receptor-associated kinase 1 (IRAK1), tumor necrosis factor receptor-associated factor-6 (TRAF6), and phosphorylation of nuclear factor-kappa B (pNF-κB) in irradiated and LPS-stimulated LX-2 cells. To our knowledge, this is the first study to show that circTUBD1 acts as a miR-146a-5p sponge to affect the viability and pro-inflammatory cytokine production of LX-2 cells through the TLR4 pathway, suggesting that circTUBD1 is a potential target for RILD therapy. ? 2020 by Radiation Research Society. All rights of reproduction in any form reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082753009&doi=10.1667%2fRR15550.1&partnerID=40&md5=a803df3f2698c882058b60e486930b55 https://scholars.lib.ntu.edu.tw/handle/123456789/485619 |
ISSN: | 0033-7587 | DOI: | 10.1667/RR15550.1 | SDG/Keyword: | circular ribonucleic acid; circular ribonucleic acid TUBD1; immunoglobulin enhancer binding protein; interleukin 1; interleukin 1beta; interleukin 6; interleukin receptor associated kinase 1; microRNA; microRNA 146a 5p; phosphotransferase; small interfering RNA; toll like receptor 4; tumor necrosis factor; tumor necrosis factor receptor associated factor 6; unclassified drug; cytokine; microRNA; MIRN146 microRNA, human; TLR4 protein, human; toll like receptor 4; TUBD1 protein, human; tubulin; apoptosis; Article; cell proliferation; cytokine production; gene expression; hepatic stellate cell; human; human cell; priority journal; biosynthesis; cell survival; genetics; liver; liver disease; metabolism; pathology; radiation induced malformation; radiation response; signal transduction; Abnormalities, Radiation-Induced; Cell Survival; Cytokines; Hepatic Stellate Cells; Humans; Liver; Liver Diseases; MicroRNAs; RNA, Circular; Signal Transduction; Toll-Like Receptor 4; Tubulin |
Appears in Collections: | 腫瘤醫學研究所 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.