Rapid and Safe Isolation of Human Peripheral Blood B and T Lymphocytes through Spiral Microfluidic Channels
Journal
Scientific Reports
Journal Volume
9
Journal Issue
1
Date Issued
2019
Author(s)
Abstract
Peripheral blood lymphocytes (PBLs) are mature lymphocytes that circulate in the blood rather than being localized to organs. A reliable label-free collection approach that can viably and appropriately isolate PBLs to establish in vitro culture systems is crucial for basic research and clinical requirements. However, isolation of PBLs from whole blood is difficult, and so the development of a rapid and safe method to perform this task is required. Microfluidic technology offers opportunities that challenge the performance of macroscale methods. In this study, we proposed a simple spiral microfluidic chip for efficient and high-throughput isolation of lymphocytes from a sample with prelysed RBCs. This spiral microfluidic platform does not rely on antibodies or biological markers for labeling cells of interest while isolating lymphocytes but rather enriches B and T lymphocytes through the different physical properties that are intrinsic to lymphocytes and other blood cells. The device was used to achieve high-throughput (~1.3 × 105 cells/min) separation of lymphocytes with high viability (>95%). Compared with previous approaches, our device provided rapid, label-free, high-throughput, and safe lymphocyte separation.
SDGs
Other Subjects
B lymphocyte; biomedical engineering; cell separation; cell survival; colorimetry; cytology; devices; equipment design; flow cytometry; human; lab on a chip; lymphocyte count; microfluidic analysis; microfluidics; normal human; procedures; T lymphocyte; treatment outcome; viscosity; B-Lymphocytes; Biomedical Engineering; Cell Separation; Cell Survival; Colorimetry; Equipment Design; Flow Cytometry; Healthy Volunteers; Humans; Lab-On-A-Chip Devices; Lymphocyte Count; Microfluidic Analytical Techniques; Microfluidics; T-Lymphocytes; Treatment Outcome; Viscosity
Publisher
NATURE PUBLISHING GROUP