Blood Plasma Separation Using a Fidget-Spinner
Journal
Analytical Chemistry
Journal Volume
91
Journal Issue
2
Pages
1247-1253
Date Issued
2019
Author(s)
Liu, C.-H.
Chen, C.-A.
Chen, S.-J.
Tsai, T.-T.
Chu, C.-C.
Chang, C.-C.
Abstract
In this study, we present a simple, hand-powered, and electricity-free centrifuge platform based on a commercially available "fidget-spinner." The centrifugal force provided by this inexpensive and easy-to-use toy is sufficient to separate whole blood, producing a plasma yield rate and purity of 30% and 99%, respectively, separated in as little as 4-7 min. We verified the separated plasma by performing a paper-based HIV-1 p24 capsid protein enzyme-linked immunosorbent assay, which achieved a recovery rate of up to 98%, indicating the plasma features extremely low matrix interference effects. These results demonstrate the reliability of the platform for practical use, in addition to greatly reducing the overall cost and time of analysis while retaining detection precision, making it suitable for medical applications in resource-limited regions of the world. ? 2018 American Chemical Society.
SDGs
Other Subjects
Blood; Medical applications; Reliability analysis; Blood-plasma separation; Capsid proteins; Centrifugal Forces; Detection precision; Enzyme linked immunosorbent assay; Matrix interference; Plasma features; Recovery rate; Separation
Type
journal article