Enhanced detection of fluorescent nanospheres using twochannel radially polarized surface plasmon microscopy
Journal
Optics Letters
Journal Volume
35
Journal Issue
17
Pages
2873-2875
Date Issued
2010
Author(s)
Abstract
We detected single dye-stained latex nanospheres as small as 20 nm using a two-detection channel modified surface plasmon microscope. We found that a radially polarized incident beam leading to excitation of well-focused surface plasmons induces both fluorescence and elastic linear scattering from the spheres. The two complementary emitted signals were detected in parallel by the two separated channels, leading to well-colocalized images. We obtained high spatial resolutions for both channels down to 250 nm in the lateral direction and 300 nm along the longitudinal axis. We believe this multimodal microscope can be useful to track nano objects and to compensate for intermittent fluorescence, thanks to a permanently activated parallel scattering detection channel.
Type
journal article