Efficient Derivation of Embryonic Stem Cells from Nuclear Transfer and Parthenogenetic Embryos Derived from Cryopreserved Oocytes
Journal
Cellular Reprogramming
Journal Volume
12
Journal Issue
2
Pages
203-211
Date Issued
2010
Author(s)
Sung, Li-Ying
Chang, Ching-Chien
Amano, Tomokazu
Lin, Chih-Jen
Amano, Misa
Treaster, Stephen B.
Xu, Jie
Chang, Wei-Fang
Nagy, Zsolt Peter
Yang, Xiangzhong
Abstract
Deriving histocompatible embryonic stem (ES) cells by somatic cell nuclear transfer (SCNT) and parthenogenetic activation (PA) requires fresh oocytes, which prevents their applications in humans. Here, we evaluated the efficiency of deriving ES cells from mature metaphase II (MII) and immature metaphase I (MI) vitrified oocytes, by PA or SCNT, in a mouse model. We successfully generated ES cell lines from PA (MII and MI) and SCNT (MII and MI) blastocysts. These cell lines expressed genes and antigens characteristic of pluripotent ES cells and produced full-term pups upon tetraploid embryo complementation. This study established an animal model for efficient generation of patient-specific ES cell lines using cryopreserved oocytes. This is a major step forward in the application of therapeutic cloning and parthenogenetic technology in human regenerative medicine and will serve as an important alternative to the iPS cell technology in countries/regions where these technologies are permitted. ? 2010, Mary Ann Liebert, Inc.
Other Subjects
animal cell; animal experiment; article; blastocyst; cell nucleus transplantation; controlled study; cryopreservation; embryonic stem cell; gene expression; metaphase; mouse; nonhuman; oocyte; parthenogenesis; pluripotent stem cell; priority journal; tetraploidy; Animals; Blastocyst; Cloning, Organism; Cryopreservation; Embryo Culture Techniques; Embryonic Stem Cells; Female; Male; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Nuclear Transfer Techniques; Oocytes; Parthenogenesis; Regenerative Medicine; Animalia; Ips
Type
journal article