https://scholars.lib.ntu.edu.tw/handle/123456789/487960
標題: | Efficient Derivation of Embryonic Stem Cells from Nuclear Transfer and Parthenogenetic Embryos Derived from Cryopreserved Oocytes | 作者: | Sung, Li-Ying Chang, Ching-Chien Amano, Tomokazu Lin, Chih-Jen Amano, Misa Treaster, Stephen B. Xu, Jie Chang, Wei-Fang Nagy, Zsolt Peter Yang, Xiangzhong LI-YING SUNG CHIH-JEN LIN |
公開日期: | 2010 | 卷: | 12 | 期: | 2 | 起(迄)頁: | 203-211 | 來源出版物: | Cellular Reprogramming | 摘要: | Deriving histocompatible embryonic stem (ES) cells by somatic cell nuclear transfer (SCNT) and parthenogenetic activation (PA) requires fresh oocytes, which prevents their applications in humans. Here, we evaluated the efficiency of deriving ES cells from mature metaphase II (MII) and immature metaphase I (MI) vitrified oocytes, by PA or SCNT, in a mouse model. We successfully generated ES cell lines from PA (MII and MI) and SCNT (MII and MI) blastocysts. These cell lines expressed genes and antigens characteristic of pluripotent ES cells and produced full-term pups upon tetraploid embryo complementation. This study established an animal model for efficient generation of patient-specific ES cell lines using cryopreserved oocytes. This is a major step forward in the application of therapeutic cloning and parthenogenetic technology in human regenerative medicine and will serve as an important alternative to the iPS cell technology in countries/regions where these technologies are permitted. ? 2010, Mary Ann Liebert, Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/487960 | ISSN: | 2152-4971 | DOI: | 10.1089/cell.2009.0072 | SDG/關鍵字: | animal cell; animal experiment; article; blastocyst; cell nucleus transplantation; controlled study; cryopreservation; embryonic stem cell; gene expression; metaphase; mouse; nonhuman; oocyte; parthenogenesis; pluripotent stem cell; priority journal; tetraploidy; Animals; Blastocyst; Cloning, Organism; Cryopreservation; Embryo Culture Techniques; Embryonic Stem Cells; Female; Male; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Nuclear Transfer Techniques; Oocytes; Parthenogenesis; Regenerative Medicine; Animalia; Ips |
顯示於: | 資訊工程學系 |
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