The associations of LPIN1 gene expression in adipose tissue with metabolic phenotypes in the chinese population
Journal
Obesity
Journal Volume
18
Journal Issue
1
Pages
7-12
Date Issued
2010
Author(s)
Abstract
The LPIN1 gene, encoding lipin-1 protein, plays critical roles in adipocyte differentiation and lipid metabolism. This study aimed to analyze the association of LPIN1 mRNA levels in human adipose tissue with metabolic phenotypes. We also examined the association of LPIN1 genetic variation with type 2 diabetes and related metabolic phenotypes in the Chinese population. The relative LPIN1 mRNA levels were measured in abdominal visceral (VAT) and subcutaneous adipose tissue (SAT) obtained from 102 nondiabetic Chinese females. Seven single-nucleotide polymorphisms (SNPs) spanning from the 5′-upstream region to the 3′-end of the LPIN1 gene were genotyped in 1,520 Chinese (760 type 2 diabetic cases and 760 controls). LPIN1 mRNA levels in VAT were negatively correlated with BMI (r = 0.21, P = 0.03), body fat percentage (r = 0.22, P = 0.02), plasma triglycerides levels (r = 0.21, P = 0.03), and plasma leptin levels (r = 0.63, P = 0.0002). LPIN1 mRNA levels were positively correlated with PPARG and ADIPOQ mRNA levels in both VAT and SAT. No single SNP of the LPIN1 gene was associated with type 2 diabetes in our population. One rare haplotype showed a significant association with type 2 diabetes (odds ratio (OR), 4.35; 95% confidence interval, 1.86-11.75; P = 4 × 10 4). No SNP or haplotype of the LPIN1 gene was associated with quantitative metabolic traits in the nondiabetic subjects. The results confirmed the association of LPIN1 gene expression in adipose tissue with lower adiposity and favorable metabolic profiles in the Chinese population. However, the LPIN1 gene seemed not to be a major susceptibility gene for type 2 diabetes or related metabolic phenotypes in the Chinese population.
SDGs
Other Subjects
adiponectin; insulin; lipid; LPIN1 protein, human; messenger RNA; nuclear protein; peroxisome proliferator activated receptor gamma; adipose tissue; adult; article; Asian; blood; body mass; China; enzyme linked immunosorbent assay; female; genetic predisposition; genetic variability; genetics; glucose blood level; haplotype; human; male; metabolism; middle aged; non insulin dependent diabetes mellitus; obesity; phenotype; reverse transcription polymerase chain reaction; risk; single nucleotide polymorphism; waist circumference; Adiponectin; Adipose Tissue; Adult; Asian Continental Ancestry Group; Blood Glucose; Body Mass Index; China; Diabetes Mellitus, Type 2; Enzyme-Linked Immunosorbent Assay; Female; Genetic Predisposition to Disease; Genetic Variation; Haplotypes; Humans; Insulin; Lipids; Male; Middle Aged; Nuclear Proteins; Obesity; Odds Ratio; Phenotype; Polymorphism, Single Nucleotide; PPAR gamma; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Waist Circumference
Type
journal article