https://scholars.lib.ntu.edu.tw/handle/123456789/495152
標題: | Novobiocin sensitizes BCRP/MXR/ABCP overexpressing topotecan-resistant human breast carcinoma cells to topotecan and mitoxantrone | 作者: | CHIH-HSIN YANG YAO-CHANG CHEN Kuo M.-L. |
公開日期: | 2003 | 卷: | 23 | 期: | 3 B | 起(迄)頁: | 2519-2523 | 來源出版物: | Anticancer Research | 摘要: | Background: Novobiocin was shown to sensitize cancer cells to etoposide and alkylating agents. Human breast carcinoma cells exposed to topotecan (MCF7/TPT300 cells) developed resistance to both mitoxantrone and topotecan. An ATP-binding cassette family protein BCRP/MXR/ABCP was overexpressed in MCF7/TPT300 cells. In addition, topotecan efflux was markedly enhanced in the resistant cells. To investigate the possibility that novobiocin may enhance cytotoxicity in BCRP/MXR/ABCP overexpressing cells, we exposed MCF7/TPT300 cells to novobiocin. Materials and Methods: Cytotoxicity tests of topotecan and mitoxantrone, as well as topotecan accumulation tests, were performed with or without novobiocin in MCF7/TPT300 cells. Results: Novobiocin enhances topotecan and mitoxantrone toxicity in MCF7/TPT300 cells at a clinically relevant concentration. Novobiocin cellular accumulation of topotecan and inhibited topotecan efflux in MCF7/TPT300 cells. Conclusion: Novobiocin may enhance topotecan and mitoxantrone toxicity in topotecan-resistant breast carcinoma cells. Novobiocin may be useful to reverse topotecan or mitoxantrone resistance in the clinic. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042807572&partnerID=40&md5=52b60e7fa0b77b29265f80867247f2d3 https://scholars.lib.ntu.edu.tw/handle/123456789/495152 |
ISSN: | 0250-7005 | SDG/關鍵字: | BCRP protein; breast cancer resistance protein; gene product; mitoxantrone; novobiocin; sulforhodamine B; topotecan; unclassified drug; article; breast cancer; concentration response; controlled study; drug accumulation; drug cytotoxicity; drug effect; drug mechanism; drug potentiation; drug transport; gene overexpression; human; human cell; priority journal; tumor cell line; Anti-Bacterial Agents; Antineoplastic Combined Chemotherapy Protocols; ATP-Binding Cassette Transporters; Breast Neoplasms; Drug Resistance, Neoplasm; Drug Synergism; Humans; Mitoxantrone; Neoplasm Proteins; Novobiocin; Topotecan; Tumor Cells, Cultured |
顯示於: | 腫瘤醫學研究所 |
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